新型纳米载体PEI-Fe_3O_4在鼻咽癌细胞基因转染中的应用  

Application of PEI-Fe_3O_4 Nanoparticles as Novel Carrier in Gene Transfection in Nasopharyngeal Carcinoma

作　　者：邓雯[1] 吴维敏[2] 亓立峰[3] 赵厚育[4] 

机构地区：[1]贵州医科大学,贵州贵阳550004 [2]同济大学医学院附属同济医院妇产科,上海200000 [3]同济大学医学院纳米院,上海200000 [4]贵州医科大学附院耳鼻喉科,贵州贵阳550004

出　　处：《贵阳医学院学报》2016年第4期382-386,共5页Journal of Guiyang Medical College

基　　金：国家自然科学基金地区科学基金项目(NO.81260353)

摘　　要：目的:构建聚乙烯亚胺(PEI)修饰的四氧化三铁(Fe_3O_4)磁性纳米粒,观察其表征,探讨其作为基因载体的可行性。方法:用PEI-Fe_3O_4磁性纳米粒包被质粒转染鼻咽癌CNEII细胞,转染miRNA-HIF-1α干扰质粒和miRNA-NC对照质粒分别为H组、NC组,空白对照组设为C组;用电子显微镜观察H组和NC组纳米粒的大小及表征,用荧光显微镜观察H组和NC组转染效率,用Real Time RT-PCR及Western Blot方法检测低氧诱导因子-1α(HIF-1α)mRNA及蛋白表达的抑制情况。结果:成功构建PEI-Fe_3O_4磁性纳米粒;H组和NC组的转染率分别为(63.6±4.2)%、(65.8±6.1)%,差异无统计学意义(P>0.05);H组HIF-1αmRNA及蛋白表达抑制率与NC组、C组比较,差异具有统计学意义(P<0.05)。结论:PEI-Fe_3O_4磁性纳米粒是一种较为理想的基因转染载体。Objective： To construct PEI-Fe3O4 nanoparticles and observe its characteristics,and to investigate the feasibility of PEI-Fe3O4 nanoparticles as carrier in transfection. Methods： Plasmids coated with PEI-Fe3O4 magnetic nanoparticels were transfected into CNEII cells,cells transfected with miRNA-HIF-1α plasmid as group H while cells transfected with miRNA-NC plasmid as NC group,and CNEII cells without transfection served as C group. Observing size and morphology of PEI-Fe3O4 nanoparticles with scanning electronic microscope. Using fluorescence microscope to measure efficiency of transfection. Proteins were detected by western blot and mRNAs were detected by RT-PCR. Results：PEI-Fe3O4 was successfully constructed. The transfection efficiency of group H and group NC was（ 63. 6 ± 4. 2） % and（ 65. 8 ± 6. 1） %,difference was not statistically significant（ P 〉0. 05）. The difference of suppression ratio of protein and mRNA of HIF-1α was significant compared to group C and group NC（ P〈 0. 05）. Conclusion： PEI-Fe3O4 magnetic nanoparticles can be used as an ideal carrier of gene transfection.

关 键 词：乙烯亚胺-四氧化三铁 磁性纳米粒 基因转染 鼻咽癌 缺氧诱导因子-1Α 信使RNA 

分 类 号：R34-33[医药卫生—基础医学] R739.6