新疆枸杞种质资源遗传多样性的SRAP分析  

Analysis of Genetic Diversity of Germplasm Resources of Lycium barbarum L.Revealed by SRAP in Xinjiang of China

作　　者：查美琴[1] 赵玉玲 李疆[1] 朱瑜[1] 罗淑萍[1] 胡月[1] 

机构地区：[1]新疆农业大学林学与园艺学院,乌鲁木齐830052 [2]新疆精河县枸杞管理中心,新疆精河833300

出　　处：《西北植物学报》2016年第4期681-687,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家林业公益性行业科研专项(201304701-1);新疆自治区果树学重点学科基金资助

摘　　要：利用SRAP分子标记技术对新疆枸杞种质资源遗传多样性及亲缘关系进行研究,为新疆枸杞种质资源分类和杂交育种提供理论依据。结果显示：（1）筛选出的12对SRAP引物共扩增出310个位点,其中多态性位点264个,多态性比率平均为84.61%,引物多态性信息含量（PIC）变化范围为0.76~0.93,平均为0.83;观测等位基因（Na）、有效等位基因数（Ne）、Neis基因多样性指数（H）、Shannon信息指数（I）平均值分别为1.846 1、1.386 9、0.228 0和0.352 0。（2）聚类分析表明,供试材料间的遗传相似系数为0.590 3~0.903 2,在遗传相似性系数为0.70和0.76时,可将30份样品分别分为2大类和5个亚类;主坐标分析结果和聚类结果基本一致。研究表明,新疆枸杞种质资源遗传多样性较丰富,且野生种与栽培品种（系）间的遗传差异较大,表现出较远的亲缘关系,而栽培品种（系）间的遗传差异相对较小,表现出较近的亲缘关系。SRAP markers were used to study the genetic diversity and phylogenetic relationship for pro- vidind a theoretical basis for identification and crossbreeding of genetic resources of Lycium barbarum L. in Xinjiang of China. The results are： （1）a total of 310 loci were produced by 12 pairs of SRAP primers, and polymorphic loci were 264 which accounted for 84. 61% in the total amplified fragments. The polymor- phism information content （PIC） values of these markers varied from 0.76 to 0.93 with an average of 0. 83. Mean values of observed number of alleles（Na）,effective number of alleles （Ne）,Nei＇s gene diversity （H） and Shannon＇s information index（I） were 1. 846 1, 1. 386 9, 0. 228 0 and 0. 352 0. （2）Range of genet- ic similarity （GS） was 0. 590 3 to 0. 903 2 among 30 wolfberry samples. Cluster analysis showed that sam- ples could be divided into 2 groups and 5 subgroups accordingly at the GS of O. 70 and 0.76. The principal coordinate analysis and clustering results are basically consistent. Research shows the genetic diversity of germplasm resources of L. barbarum L. in Xinjiang was relatively abundant. The genetic difference be-tween wild species and cultivars （lines） was relatively large,show a distant relationship, and the genetic difference among cultivars （lines） was relatively small, show a close relationship.

关 键 词：枸杞 相关序列扩增多态性(SRAP) 聚类分析 主坐标分析 遗传多样性 

分 类 号：Q346.5[生物学—遗传学]