艾叶提取物的化学成分及抗HBV活性分析  

Chemical Constituents of Artemisiae Argyi Folium Extract and Its Anti-HBV Activity Screening

作　　者：赵志鸿[1] 吴芳[2] 郑立运[1] 王丽阳[2] 侯迎迎[2] 耿楠[2] 王桂芳[1] 

机构地区：[1]郑州大学医药科学研究院,河南省肝病药理重点实验室,郑州450052 [2]郑州大学药学院,郑州450001

出　　处：《中国实验方剂学杂志》2016年第9期30-34,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省重点科技攻关计划项目(122102310651)

摘　　要：目的：研究菊科艾Artemisia argyi干燥叶的化学成分及抗乙型肝炎病毒（HBV）活性。方法：采用硅胶柱色谱分离其化学成分,并根据理化性质、核磁共振波谱法、质谱法以及参考相关文献鉴定化合物结构,采用噻唑蓝（MTT）法测定化合物对Hep G 2.2.15细胞的毒性以及酶联免疫（ELISA）法检测各组分对Hep G 2.2.15细胞上清液中HBe Ag和HBs Ag分泌的影响。结果：从艾叶乙酸乙酯部位分离鉴定了5个化合物,分别为豆甾醇（1）,5,4＇-二羟基-6,7,3＇-三甲氧基黄酮（3＇-甲氧基蓟黄素）（2）,达玛二烯醇乙酸酯（3）,5,7,4＇-三羟基-3＇,8-二甲氧基黄酮（4）,十六烷酸丙酯（5）。结论：化合物2～4为首次从艾叶中分离得到,其中3＇-甲氧基蓟黄素对HBs Ag和HBe Ag有抑制作用,其对HBs Ag的半数抑制浓度（IC50）为8.09 mg·L-1,对HBe Ag的IC50小于2.5 mg·L-1。Objective： To study the chemical components of Artemisiae Argyi Folium and their antihepatitis B virus（ HBV） activities. Method： The chemical components were isolated and purified by silica gel column chromatography,and their structures were identified according to their physicochemical properties,nuclear magnetic resonance spectroscopy（ NMR）, mass-spectrography（ MS） and literature analysis. Furthermore, the toxicity of these chemical components to Hep G 2. 2. 15 cells was measured by MTT method and their effects on HBe Ag and HBs Ag secretion in Hep G 2. 2. 15 cell supernatant were measured by ELISA method. Result： Five compounds were isolated from Artemisiae Argyi Folium and their structures were identified as stigmasterol（ 1）,5,4＇-dyhydroxyl-6,7,3＇-trimethoxyflavone（ 3＇-methoxythistle flavin）（ 2）, dammaradienylacetate（ 3）,5,7,4＇-trihydroxy-3＇,8-dimethoxyflavone（ 4）,and hexadecanoic dihydro ypropyleste（ 5）. Conclusion： Compounds 2-4were isolated from Artemisiae Argyi Folium for the first time and 3＇-methoxythistle flavin showed inhibitory effect on HBs Ag and HBe Ag lines with IC50 values of 8. 09 mg·L-1 and less than 2. 5 mg·L-1 respectively.

关 键 词：艾叶 化学成分 分离纯化 结构鉴定 细胞活性 乙型肝炎病毒 

分 类 号：R284.1[医药卫生—中药学]