UPLC同时检测加味逍遥提取物6种指标性成分含量  

Simultaneous Determination of Six Components in Extract of Modified Xiaoyao by UPLC

作　　者：李明月[1] 周立红[2] 张文生[2] 章顺楠[2] 叶正良[3] 柳文媛[1] 

机构地区：[1]中国药科大学,南京210009 [2]天士力制药集团股份有限公司,天津300410 [3]天津天士力之骄药业有限公司,天津300410

出　　处：《中国实验方剂学杂志》2016年第9期58-62,共5页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立同时测定加味逍遥提取物中6个指标性成分(栀子苷、芍药内酯苷、芍药苷、甘草苷、苯甲酰芍药苷、甘草酸)含量方法。方法:采用超高效液相色谱法,以UPLC^(TM)HSS T_3色谱柱(2.1 mm×100 mm,1.8μm),乙腈-0.05%磷酸水溶液为流动相梯度洗脱,柱温30℃,体积流量0.4 m L·min^(-1),检测波长230 nm。结果:6个指标性成分分离度良好,均在各自线性范围内线性关系良好,R2≥0.999 4;平均加样回收率为98.29%~101.67%,RSD均<3.8%。结论:该法准确、简便、分离度良好,可作为加味逍遥提取物的质量评价方法。Objective： To establish an UPLC method for the simultaneous determination of geniposide,albiflorin,paeoniflorin, liquiritin, benzoylpaeoniflorin, and glycyrrhizic acid in extract of modified Xiaoyao extract. Method： UPLC was performed on UPLCTMHSS T3column（ 2. 1 mm × 100 mm,1. 8 μm） with acetonitrile-0. 05% phosphoric acid solution as the mobile phase for gradient elution. The detection wavelength was set at 230 nm. The flow rate was 0. 4 m L·min-1 and column temperature was 30 ℃. Result： The six components were well separated and showed good linearity in their respective concentration ranges with R2≥ 0. 999 4. The average recovery was 98. 29%-1 01. 67% with RSD less than 3. 8%. Conclusion： The established method is simple and stable with satisfactory separation degree. It can be used for the quality control of modified Xiaoyao extract.

关 键 词：加味逍遥提取物 超高效液相色谱法 栀子苷 芍药内酯苷 芍药苷 甘草苷 苯甲酰芍药苷 甘草酸 

分 类 号：R284.1[医药卫生—中药学]