基于对植物神经系统相关指标影响的洋金花各化学拆分组分的药性研究  

Medicinal Properties of Chemical Components in Daturae Flos Based on Effects on Relevant Indicators of Autonomic Nervous System

作　　者：李振宇[1] 王秋红[1] 杨炳友[1] 舒尊鹏[1] 匡海学[1] 

机构地区：[1]黑龙江中医药大学北药基础与应用研究省部共建教育部重点实验室,黑龙江省中药及天然药物药效物质基础研究重点实验室,哈尔滨150040

出　　处：《中国实验方剂学杂志》2016年第9期86-90,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展计划(973计划)项目(2006CB504708,2013CB531801);黑龙江中医药大学优秀青年教师支持计划项目(051282)

摘　　要：目的:进一步阐明洋金花化学拆分组分的药性归属,为"中药一味一性,一药X味Y性(Y≤X)"的中药性味理论新假说的客观性提供科学依据。方法:取雄性昆明种小鼠和Wistar大鼠,分别随机分为正常组,洋金花水煎液组,生物碱组分高、低剂量组,大孔树脂50%乙醇洗脱组分高、低剂量组。各组分别ig给药,正常组给同体积生理盐水,每天给药1次,连续10 d。观察洋金花及其化学拆分组分对植物神经系统相关的心率、呼吸频率、血压、唾液分泌量、体温指标的影响,作为洋金花药性评价系统的一部分,对洋金花及其化学拆分组分的药性做进一步评价。结果:与正常组比较,洋金花及生物碱组分明显使小鼠心率增加、大鼠呼吸频率加快、大鼠动脉平均血压升高、小鼠唾液分泌抑制、大鼠体温升高(P<0.05,P<0.01)。大孔树脂50%乙醇洗脱组分可促进小鼠唾液分泌,对小鼠心率减少、大鼠呼吸频率减缓有一定作用趋势(P<0.05,P<0.01),对大鼠动脉平均血压和体温无明显作用。大孔树脂50%乙醇洗脱组分与生物碱组分比较,对小鼠心率,大鼠呼吸频率、大鼠动脉平均血压、小鼠唾液分泌量、大鼠体温影响有一定差异或差异具统计学意义(P<0.05,P<0.01)。结论:洋金花及生物碱组分药性为温,大孔树脂50%乙醇洗脱组分与生物碱组分药性不同,结合前期研究结果,可判定其药性为凉。Objective： To clarify the medicinal properties of chemical components in Daturae Flos,and provide scientific basis for the objectivity of new hypothesis theory ＂One flavor and one property for Chinese medicine,X flavors and Y properties for one medicine（ Y≤X） ＂. Method： Male Kunming mice and Wistar rats were randomly divided into normal group,Daturae Flos decoction group,alkaloid components high dose group,low dose group,macroporous resin 50% ethanol elution components high dose group and low dose group. The mice were treated by intragastric administration and the mice in normal group received same volume of normal saline,1time a day for consecutive 10 days. The effects of Daturae Flos and its chemical components on heart rate,breathing rate,blood pressures, the amount of salivary secretory and body temperature relative to autonomic nervous system were observed as part of the medicinal properties evaluation of Daturae Flos for further research.Result： Compared with the normal group,the Daturae Flos and its alkaloid components had certain effect or effect trend in increasing the heart rate of mice,accelerating the breathing rate of rats,increasing the mean arterial blood pressure in rats,inhibiting salivary secretory in mice and increasing body temperature of rats（ P〈0. 05,P〈0. 01）. Macroporous resin 50% ethanol elution components could promote salivary secretory in mice,and have certain effect on reducing heart rate of mice and reducing breathing rate of rats（ P〈0. 05,P〈0. 01）,but with no significant effect on mean arterial blood pressure or body temperature in rats. There was certain difference or statistically significant difference between macroporous resin 50% ethanol elution components and alkaloid components in effects on heart rate of mice,breathing rate of rats,mean arterial blood pressure in rats,salivary secretory in mice and body temperature of rats（ P〈0. 05,P〈0. 01）. Conclusion： Daturae Flos and its alkaloid components have warm medicinal properties,while macr

关 键 词：洋金花 中药药性 化学拆分组分 植物神经系统 

分 类 号：R285.5[医药卫生—中药学]