丹皮酚与三七总皂苷配伍应用对血管紧张素Ⅱ诱导增殖的心脏成纤维细胞Ⅰ型和Ⅲ型胶原蛋白表达的影响  

Effects of Combination of Paeonol and Notoginseng Radix et Rhizoma Saponins on Expressions of Type Ⅰ and Ⅲ Collagen in Proliferation of Cardiac Fibroblasts Induced by Angiotensin Ⅱ

作　　者：孙红丹[1] 聂丹[1] 龚明玉[1] 周晓慧[1] 

机构地区：[1]承德医学院,河北承德067000

出　　处：《中国实验方剂学杂志》2016年第9期122-126,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河北省自然科学基金项目(C2011406009);河北省教育厅重点项目(ZD20131068);河北省教育厅项目(Q2012015)

摘　　要：目的:探讨丹皮酚与三七总皂苷配伍应用对血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)诱导增殖的心脏成纤维细胞胶原合成的调节作用。方法:选取原代培养的乳鼠心脏成纤维细胞,随机分为6组,分别为空白组,模型组,丹皮酚(45 mg·L^(-1))组,三七总皂苷(50 mg·L^(-1))组,丹皮酚与三七总皂苷配伍的高、低剂量(丹皮酚45,30 mg·L^(-1)+三七总皂苷50,30 mg·L^(-1))组。药物干预48 h,采用逆转录-聚合酶链式反应(RT-PCR)法检测Ⅰ型胶原,Ⅲ型胶原的mRNA表达水平,蛋白质免疫印迹(Western blot)法检测各组细胞的Ⅰ型胶原和Ⅲ型胶原的蛋白表达水平。结果:与空白组比较,模型组Ⅰ型胶原和Ⅲ型胶原的mRNA及蛋白表达明显升高(P<0.01);与模型组比较,AngⅡ可刺激心脏成纤维细胞(cardiac fibroblasts,CFs)的增殖,丹皮酚,三七总皂苷及其配伍的高、低剂量均可抑制Ⅰ型胶原和Ⅲ型胶原的mRNA及蛋白表达(P<0.05),其中配伍组的抑制作用较优,且配伍高剂量作用时抑制效果更佳。结论:丹皮酚和三七总皂苷配伍组方可抑制血管紧张素Ⅱ诱导增殖的心脏成纤维细胞Ⅰ型胶原和Ⅲ型胶原蛋白的基因表达,显示明显的协同效应。Objective： To investigate the effect of combination of paeonol（ Pae） and Notoginseng Radix et Rhizoma saponins（ NRS） on expressions of type Ⅰ and Ⅲ collagen in proliferation of cardiac fibroblasts induced by angiotensin Ⅱ（ AngⅡ）. Method： Primary cultured cardiac fibroblasts（ CFs） of neonatal rats were randomly divided into six groups： blank group, model group, Pae group（ 45 mg ·L-1）,NRS group（ 50 mg·L-1）,combined Pae and NRS high-dose and low-dose groups（ Pae 45,30 mg·L-1＋ NRS 50,30 mg·L-1）. The mRNA expressions of type Ⅰ and Ⅲ collagen were detected by RT-PCR after drug intervention for 48 h. The protein expressions of type Ⅰ and Ⅲ collagen were determined by Western blot. Result： As compared with the blank group,the mRNA expressions and protein expressions of type Ⅰ and Ⅲ collagen were significantly increased in model group（ P〈0. 01）. Compared with the model group,the proliferation of CFs were stimulated by AngⅡ,and the mRNA and protein expressions of type Ⅰ and Ⅲ collagen were inhibited by Pae group,NRS group,and the combined Pae and NRS high-dose and low-dose groups（ P〈0. 05）. The combined groups had better efffect than other groups,and the combined high-dose group had the best inhibitory effect. Conclusion： The combination of Pae and NRS can inhibit the gene expressions of type Ⅰ and Ⅲ collagen in cardiac fibroblasts induced by AngⅡ,and shows significant synergistic effects.

关 键 词：丹皮酚 三七总皂苷 心脏成纤维细胞 胶原 

分 类 号：R285.5[医药卫生—中药学]