一个融合魏斯氏菌隐蔽质粒的序列分析  

Complete DNA Sequence Analysis of a Cryptic Plasmid Isolated from Weissella confusa

作　　者：王海娟[1] 戴雨珂 苏森森[1] 王英[3] 潘渠[1] 

机构地区：[1]成都医学院病原生物学教研室,成都610500 [2]成都医学院公共卫生系,成都610500 [3]成都军区总医院肾内科,成都610500

出　　处：《生物技术通报》2016年第4期217-221,共5页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31170007);四川省教育厅科技基金重点项目(14ZA0219);成都医学院病原生物学学科建设项目(CYXK2012005)

摘　　要：从融合魏斯氏菌(Weissella confusa QJ012)中发现一个隐蔽质粒(p QJ012),测序结果显示该质粒大小为1489 bp,碱基G+C含量为42.8%。BLAST结果显示该质粒的核苷酸序列同pJY33和pKLCA的同源性分别为93%、92%。ORF1编码一个282个氨基酸残基的复制蛋白(Rep),其氨基酸序列与质粒pJY33和pKLCA的同源性分别为91%、85%。根据复制蛋白、dso和sso的序列特征,推定pQJ012的复制方式为滚环复制,是滚环复制pT181家族成员。质粒pQJ012可能构建为良好的表达载体。A cryptic plasmid isolated from Weissella confusa QJ012, designated as pQJ012, was sequenced and characterized, and it was a 1489 bp circular molecule with a 42.8% G＋C content. The result of BLAST showed that nucleotide sequence of pQJ012 was in homology with pJY33 （ 93% ） and pKLCA （ 92% ） . ORF1 encoded a 282 amino acid residue Rep protein that was 91% identical to pJY33 and 85% identical to pKLCA in homology. Sequence analysis indicated that plasmid pQJ012 belonged to pT181 family of rolling-circle replication（ RCR ）. The pQJ012 may be constructed as a promising expression vector.

关 键 词：融合魏斯氏菌 隐蔽质粒 序列分析 复制蛋白 

分 类 号：Q78[生物学—分子生物学]