不同类型白癜风基因谱表达差异的初步研究  

Preliminary study of differential gene expression profiling between segmental vitiligo and non-segmental vitiligo

作　　者：聂慧琼 王平[1,2] 张小燕[2] 侯秀丽[1] 许文[2] 邵琼琰[3] 

机构地区：[1]安徽医科大学附属杭州临床学院皮肤科,杭州310000 [2]杭州市第三人民医院皮肤科,杭州310000 [3]浙江中医药大学附属杭州市第三人民医院皮肤科,杭州310000

出　　处：《安徽医科大学学报》2016年第5期707-711,共5页Acta Universitatis Medicinalis Anhui

基　　金：杭州市医学重点专科专病项目(编号:20140733Q19);浙江省医药卫生科技计划项目(编号:2014KYB200)

摘　　要：目的探讨白癜风临床类型与基因表达差异的相关性。方法分别选择节段型白癜风(SV)、非节段型白癜风(NSV)和健康对照(HI)各4例,应用Phalanx人全基因组表达谱芯片检测外周血淋巴细胞基因谱表达差异,并通过RTPCR进行验证。结果与HI相比,SV有239个上调基因和175个下调基因,主要涉及适应性免疫反应、细胞因子受体相互作用、趋化因子信号通路,NSV有88个上调基因和560个下调基因,涉及先天性免疫、细胞自噬与凋亡、黑素细胞生物学、泛素介导的蛋白裂解与酪氨酸酶代谢。SV和NSV共同基因表达差异包括60个上调基因和60个下调基因;相对于SV,NSV表达223个上调基因和129个下调基因,主要包括嘌呤、嘧啶、鞘脂类代谢。结论 SV有不同于NSV的遗传背景与发病机制。Objective To investigate the correlation between clinical pattern and differential gene expression in pa- tients with vitiligo. Methods Peripheral blood lymphoeytes obtained from four cases of segmental vitiligo （SV）, non-segmental vitiligo （NSV） and healthy individual （HI） respectively. Whole genome expression microarrays were used to assay the gene expression profiles between SV, NSV and HI. Quantitative PCR assay was used to vali- date the gene expression of array. Results Compared to HI, 239 over-expressed and 175 down-expressed genes were detected in SV, which were mainly involved in the adaptive immune response, cytokine-cytokine receptor in- teraction and chemokine signaling. In NSV, 88 over-expressed and 560 down-expressed genes were found and were mainly involved in the innate immune, autophagy, apoptosis, melanocyte biology, ubiquitin mediated proteolysis and tyrosine metabolism, which were different from SV. Sixty over-expressed and sixty down-expressed genes shared similar tendency in SV and NSV. Compared to SV, 223 over-regulated and 129 down-regulated genes were found in NSV, which were mainly involved in the metabolism of purine, pyrimidine and sphingolipid. Conclusion There may exit different genetic background and pathogenesis between SV and NSV.

关 键 词：白癜风 节段型 白癜风 非节段型 基因表达 

分 类 号：R75[医药卫生—皮肤病学与性病学]