Bac-to-Bac系统双表达mChIL18基因和H5亚型AIV HA1基因及其免疫原性  

Immunogenicity of double expression protein including HA1 gene from H5 AIV and mChIL-18 gene with Bac-to-Bac system1

作　　者：孔娜[1] 

机构地区：[1]菏泽学院药物科学与技术系,山东菏泽274015

出　　处：《中国兽医学报》2016年第5期712-717,共6页Chinese Journal of Veterinary Science

基　　金：菏泽学院科研基金科技计划资助项目(XY12BS04)

摘　　要：为了制备禽流感病毒(avian influenza virus,AIV)基因工程亚单位疫苗,将其HA1基因和鸡IL-18成熟蛋白基因(mChIL-18)分别或先后插入到双表达载体pFastBacTM Dual中,表达单表达或双表达蛋白pHA1、pIL18和pHA1-IL18。将表达蛋白和/或禽流感二价(H5+H7)灭活疫苗通过腿部肌肉多点注射免疫7日龄SPF鸡,2周后加强免疫1次。结果显示,pHA1-IL18、pHA1+pIL18、pIL18+禽流感疫苗、禽流感疫苗和pHA1免疫雏鸡后均能促进CD4+T、CD8+T细胞的增殖和抗体水平的增加,且pHA1-IL18免疫组要优于pHA1+pIL18混合免疫组及其他免疫组和对照组,差异显著(P<0.05)。结果表明,pHA1-IL18可以产生比pIL18+pHA1更强的免疫原性,为新型疫苗的研制奠定基础。In order to develop agenetic engineering subunit vaccine against AIV,the HA1 gene and mChIL-18 gene were inserted into the double expression plasmid pFastBacTM Dual to express the single expression protein pHA1,pIL18 and double expression protein pHA1-IL18.The 7-day-old chickens were immunized intramuscularly with recombinant expression protein,and then boosted two weeks later.The results showed that immunization with pIL18 and AIV vaccine led to only a marginal increase in antibody titer and CD4＋,CD8-＋T cell responses,while immunization with pHA1-IL18 or pHA1＋ pIL18 led to a dramatic augmentation of antibody titer and CD4-＋,CD8-＋T cell responses.However,pHA1-IL18 showed better effect than pHA1＋ pIL18,pIL18＋ AIV vaccine and pHA1.These results demonstrate that pHA1-IL18 can be potentially used as a vaccine for protection of chickens against AIV.

关 键 词：HA1基因 mChIL-18基因 pFastBacTM Dual载体 双表达 免疫原性 

分 类 号：S852.659.5[农业科学—基础兽医学]