罗格列酮对非酯化脂肪酸体外诱导的犊牛原代肝细胞线粒体功能紊乱的影响  

Effect of rosiglitazone on NEFAs-induced mitochondrial dysfunction in calf hepatocytes in vitro

作　　者：高文文[1] 王亚洲[1] 赵志波[1] 刘欢[1] 张世奇[1] 王哲[1] 李心慰[1] 刘国文[1] 

机构地区：[1]吉林大学动物医学学院,吉林长春130062

出　　处：《中国兽医学报》2016年第5期833-838,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31372494;31402265;31472247;31460681)

摘　　要：本试验旨在探讨非酯化脂肪酸（Nonestesterified fatty acid,NEFAs）对体外原代培养犊牛肝细胞线粒体功能以及罗格列酮对NEFAs诱导的线粒体功能紊乱的影响。体外添加NEFAs（1.8mmol/L）处理犊牛原代肝细胞不同时间,运用Western blot和实时荧光定量PCR（qRT-PCR）方法检测线粒体呼吸链复合体IV（COX IV）蛋白水平及mRNA水平的影响。另外选取细胞,罗格列酮（10nmol/L）预处理细胞24h后添加NEFAs（1.8mmol/L）处理9h。运用Western blot和qRT-PCR方法检测PGC-1α、Mfn-2的蛋白表达水平及COX IV mRNA表达水平。结果显示,随着NEFAs作用时间的增加,与0h组相比,NEFAs处理5h以上时COX IV蛋白表达水平极显著降低（P〈0.01）,处理7-12h时COX IV mRNA表达水平呈显著降低（P〈0.05）。添加罗格列酮后,罗格列酮可极显著增加COX IV mRNA水平,PGC-1α、Mfn-2蛋白表达水平（P〈0.01）。结果表明,NEFAs可诱导犊牛原代肝细胞发生线粒体功能紊乱,罗格列酮可缓解由NEFAs诱导的犊牛原代肝细胞线粒体功能紊乱。To investigate the effect of none-stesterified fatty acid（NEFAs）on mitochondrial function and the effect of rosiglitazone on NEFAs-induced mitochondrial dysfunction in calf hepatocytes in vitro.Calf hepatocytes were treated with NEFAs（1.8mmol/L）for different time.The level of COX IV protein and mRNA expression were examined by using Western blot and realtime fluoresence quantitative RT-PCR（qRT-PCR）.Hepatocytes were pretreated with rosiglitazone（10nmol/L）for 24 h,then NEFAs（1.8mmol/L）were added and cultured for another 9h.The level of PGC-1α,Mfn-2proteins and COX IV mRNA expression were examined by using western blot and qRT-PCR.The results showed that the level of COX IV protein decreased significantly（P〈0.01）after NEFAs treatment more than 5hcompared with no treatment group,COX IV mRNA decreased significantly（P〈0.05）in 7-12 hgroups compared with no treatment group.Rosiglitazone strongly enhanced the levels of COX IV mRNA,PGC-1αand Mfn-2proteins in NEFAs-induced mitochondrial dysfunction（P〈0.01）These results confirm that NEFAs can induce mitochondrial dysfunction and rosiglitazone could prevent NEFAs-induced mitochondrial dysfunction in calf hepatocytes,at least in part.

关 键 词：罗格列酮 非酯化脂肪酸 线粒体功能紊乱 肝细胞 奶牛 

分 类 号：S856.5[农业科学—临床兽医学]