培养条件对脱硫脱硫弧菌合成纳米硫化铅的影响  

The effect of culture condition on synthesis of nanoparticles lead sulf ide by Desulfovibrio desulfuricans

作　　者：贡俊[1] 宋霄敏 高艳[2] 白红娟[2] 王玉芬[1] 韩晋仙[1] 

机构地区：[1]山西财经大学环境经济学院,太原030006 [2]中北大学化工与环境学院,太原030051

出　　处：《应用与环境生物学报》2016年第2期206-212,共7页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(31200049);山西省青年科技基金项目(2012011009-1)资助~~

摘　　要：研究pH值、温度条件对脱硫脱硫弧菌合成硫化铅纳米产物的影响,并分别用X射线衍射、能谱和高分辨率透射电镜对产物的形貌进行分析.结果显示,在不同pH值和温度条件下,脱硫脱硫弧菌对铅离子的转化率均在98%以上,pH为7和温度为30℃时,铅的转化率达到99.7%.随着反应体系初始pH值由5增大至8,制得的纳米硫化铅由近球形逐渐转变为杆状颗粒,粒径由10-15 nm逐渐增大至15-30 nm,pH值为9时,制备出了具有特殊形貌单分散的不规则状纳米硫化铅,粒径为10-25 nm.在15-30℃下,温度的改变对产物的形貌以及尺寸大小没有明显影响,制备的纳米硫化铅粒子均呈不规则杆状,粒径大小主要分布在15-30 nm范围内.铅离子进入细胞内与硫化物结合生成纳米硫化铅,然后再释放到溶液中.综上,不同pH条件下脱硫脱硫弧菌合成了不同形貌的纳米硫化铅,温度对产品形貌影响不大,脱硫脱硫弧菌对铅离子均有较高的转化率,纳米硫化铅可以在菌体内部形成;脱硫脱硫弧菌合成纳米硫化铅产品具有简单、稳定、粒径较小等优点,可为生物合成纳米产品提供借鉴.Nanoparticles Pb S is synthesized by Desulfovibrio desulfuricans under different conditions with advantages of simplicity, stability and small particle size, which is a good example of biological synthesis of nanometer products. This research investigated the effects of processing variable changes including pH and temperature on the synthesis of nanoparticles Pb S products by D. desulfuricans. The microstructure of the Pb S nanoparticles was analyzed by XRD, EDS and HRTEM, respectively. And the mechanism was also studied. The conversion rate of Pb2＋ as over 98% under all pH and temperature conditions, up to 99.7% with pH 7 and 30 °C. With the increase of pH from 5 to 8, the shape of Pb S nanoparticles changed from spheroidal to rod and the particle sizes enlarged from 10-15 nm to 15-30 nm. The monodisperse irregular nanoparticles Pb S were produced with 10-25 nm under pH 9. The temperature change between 10 °C and 35 °C had no effect on the shape or size of nanoparticles Pb S, which kept irregular rods and 15-30 nm. Nanoparticles Pb S was produced by Pb2＋ and sulfide in the cells and released into the solution. The results indicated that the pH change may cause mor phology variation in Pb S nanoparticles, but the temperature has little impact on them. D. desulfuricans has high conversion rate of Pb2＋. Nanoparticles Pb S can be formed in the cells.

关 键 词：脱硫脱硫弧菌 纳米硫化铅 合成 PH值 温度 

分 类 号：Q939.9[生物学—微生物学]