黄鳝胃瘤线虫线粒体cox1基因的多态性研究  

Polymorphism of the Mitochondrial cox1 Gene of Eustrongylides Spp.from Monopterus Albus

作　　者：黄汉成[1] 王芝英[2] 林洁[2,3] 马光旭[2] 朱宏宏 周荣琼[2] 

机构地区：[1]西南大学(荣昌校区)动物科学系,重庆荣昌402460 [2]西南大学(荣昌校区)动物医学系,重庆荣昌402460 [3]四川省达州市动物疫病预防控制中心,四川达州635000

出　　处：《西南大学学报（自然科学版）》2016年第5期7-12,共6页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金项目(31172313)

摘　　要：为了解渝西地区黄鳝胃瘤线虫分离株线粒体细胞色素C氧化酶第I亚基（cox1）的遗传变异情况及与其他科线虫的种群遗传关系,本研究对该地区黄鳝胃瘤线虫cox1部分序列进行了克隆测序和进化分析.结果表明渝西地区6株胃瘤线虫分离株的cox1序列长度一致,均为441bp;各分离株之间的相似性为97.5%~100%.种系发育树表明,6个胃瘤线虫分离株均位于同一分枝.由于胃瘤线虫cox1序列种内相对保守,种间差异较大,故可作为种间遗传变异研究的标记.本研究结果为胃瘤线虫的分类鉴定以及进一步的分子流行病学调查和群体遗传研究奠定了基础.The study was carried out to examine the genetic variations of the mitochondrial Cytochrome C oxidase subunit 1 gene （cox1） among Eustrongylides spp. isolates of Monopterus albus in Western Chongqing area, and to investigate the phylogenetic relationship among other nematodes as well. Molecular cloning was performed to obtain the partial cox i sequence of each E. spp. sample, and the homology anal- ysis of these sequences were conducted. The results showed the same length of cox1 sequences （441 bp. ） of 6 E. spp. isolates with similarity of 97.5%--100%. Phylogenetic analysis of these sequences showed that all E. spp. isolates were grouped into a single clade. There was significant discrepancy between inter- specific sequesece and ir a-specific sequence in terms of cox1 sequence, suggesting cox1 gene could be used as genetic marker for identification. The result of this study provides scientific basis and theo- retical reference on the molecular level and epidemiology survey of parasitic Eustrongylides.

关 键 词：胃瘤线虫 线粒体DNA cox1基因 种系发育关系 

分 类 号：S917.4[农业科学—水产科学]