Cu^(2+)对离体培养的红花百里香不定芽生长及挥发油的影响  

Effects of Cu^(2+)on the Growth of the Adventitious Buds of Thyme serpyllumin in vitro Culture and Their Essential Oil

作　　者：徐治朋 李晓东[2] 鲁朝晖[2] 李凌[1] 

机构地区：[1]西南大学园艺园林学院,重庆400715 [2]深圳职业技术学院化生学院,广东深圳518055

出　　处：《西南大学学报（自然科学版）》2016年第5期37-44,共8页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市农业科技成果转化资金项目(cstc2013jcsf-nycgzhA80001)

摘　　要：以离体培养的红花百里香不定芽为材料,采用不同浓度CuCl2处理,测定不定芽的各种指标,研究Cu2+对红花百里香不定芽的增殖生长及挥发油的影响.结果表明,低浓度的CuCl2可以促进红花百里香不定芽的增殖生长,而高浓度的CuCl2起抑制作用;其中50μmol/L的CuCl2对不定芽的增殖效果最好,增殖系数高达20.35,精油含量达到27.25‰,提高了23.98%,且颜色更深.说明一定浓度的Cu2+能促进红花百里香不定芽的增殖生长,增强其抗逆性,促进百里香挥发油的合成积累,改变其成分组成.The adventitious buds of Thyme serpyllum induced in in vitro culture were treated by Cu2＋ at different concentrations, and then various indicators were measured to investigate effect of Cu2＋ on their growth and essential oil. The results showed that low concentrations of Cu2＋ facilitated the proliferation of the adventitious buds while high concentrations of Cu2T inhibited it. Of all the concentrations of CuC12 studied, the treatment of 50μmol/L gave the highest proliferation coefficient （20.35） and the highest es- sential oil content （27.25%）, which was 23.98% more than the control, and the color of the essential oil was deeper. Therefore, the study showed that the addition of Cu2＋ at a suitable concentration could pro- mote the proliferation of adventitious buds of T. serpyllum and enhance their resistance, and promote the accumulation of essential oil and change its composition.

关 键 词：红花百里香 CU2+ 可溶性糖 可溶性蛋白 SOD POD PAL 挥发油 

分 类 号：Q945.78[生物学—植物学]