柳氮磺胺吡啶对胶原诱导关节炎Th17/Treg细胞的影响  

Modulating Effects of Sulphasalazine on The Balance of Th17/Treg Cells in Collagen Induced Arthritis

作　　者：汤军[1] 丁嵩涛[1] 刘莉[1] 秦启忠[1] 彭惠民[1] 刘含登[1] 

机构地区：[1]重庆医科大学实验教学管理中心,重庆400016

出　　处：《西南大学学报（自然科学版）》2016年第5期66-70,共5页Journal of Southwest University(Natural Science Edition)

基　　金：国家高技术研究发展计划项目(863计划);863计划项目(2014AA022209);国家自然科学基金(81370906)

摘　　要：目的:评价柳氮磺胺吡啶对胶原诱导性小鼠关节炎的治疗作用,并探讨其对Th17/Treg细胞的调控作用.方法:构建胶原诱导关节炎(collagen induced arthritis,CIA)模型,II型胶原免疫小鼠,21d后将关节炎小鼠分为对照组和柳氮磺胺吡啶组,记录关节炎临床评分,观察病理切片,比较关节炎发病严重程度;流式细胞术检测脾脏淋巴细胞Th17及Treg细胞的比例.结果:柳氮磺胺吡啶能显著降低CIA小鼠关节炎的临床评分(p<0.05),脾脏Th17细胞水平(p<0.05)在一定程度上促进Treg细胞的表达.结论:柳氮磺胺吡啶调控Th17细胞与Treg细胞平衡,可能是其治疗类风湿关节炎的作用机制之一.The aim is to evaluated the effect and mechanism of sulphasalazine （SSZ） in murine model of collage-induced arthritis （CIA） on modulating the balance of Thl7/Treg cells by establishing the CIA model. After 21 days, DBA1 mice were divided into CIA and CIA ＋ SSZ group. The severity of arthritis joint was assessed by using arthritis score. The numbers of Thl7 cells and CD4 ＋ CD25 ＋ Foxp3＋ Tregs were determined by flow cytometry. In comparison with CIA group, CIA ＋SSZ group reduced the disease severity obviously （p〈0.05）. Sulphasalazine reduced Thl7 cells （p〈0.05）. In addition, SSZ has effect on Treg cells＇ number. These results indicate that SSZ effect on the balance of Th17/Treg through sup- pressing Thl7 cells, and stimulating Treg cells. Sulphasalazine may act as a potential immunomodulator for the treatment of rheumatoid arthritis （RA）.

关 键 词：类风湿关节炎 柳氮磺胺吡啶 TH17细胞 调节性T细胞 胶原诱导性关节炎 

分 类 号：Q4[生物学—生理学] R392[医药卫生—免疫学]