丹参bHLH转录因子基因SmMYC2的克隆和表达分析  

Cloning and Expression Analysis of SmMYC2,a bHLH Transcription Factor Gene from Salvia miltiorrhiza Bunge

作　　者：周文平[1] 王怀琴[1] 郭晓荣[1] 杨新兵[1] 化文平[2] 曹晓燕[1] 

机构地区：[1]陕西师范大学,药用资源与天然药物化学教育部重点实验室,西北濒危药材资源开发国家工程实验室,西安710062 [2]陕西学前师范学院生物科学与技术系,西安710061

出　　处：《植物科学学报》2016年第2期246-254,共9页Plant Science Journal

基　　金：陕西省自然科学基金项目(2014JQ3107);中央高校基本科研业务费专项资金项目(GK201302043);陕西学前师范学院基金(2014DS010)

摘　　要：MYC2是植物茉莉酸类激素响应途径中的核心转录因子,在植物防御反应、次生代谢调控及生长发育过程中均有重要的调控作用。基于丹参转录组和基因组survey序列,本研究克隆了丹参转录因子MYC2的基因序列,并命名为Sm MYC2(Gen Bank No.KJ945636)。Sm MYC2基因的c DNA序列长度为1910 bp,开放阅读框(ORF)的长度为1809 bp,编码602个氨基酸,无内含子;该基因编码蛋白与烟草、番茄等植物的MYC2蛋白具有较高的同源性;Sm MYC2蛋白无跨膜结构域、信号肽等结构。实时荧光定量PCR检测结果显示,Sm MYC2在丹参的根、茎、叶、花中均有表达,并且在根和茎中的表达量更高;此外,该基因表达受茉莉酸甲酯、光和机械损伤的诱导,但受赤霉素的抑制。本实验结果为进一步探讨Sm MYC2基因在丹参中的生物学功能奠定了基础。MYC2 is a core transcription factor in the response procedure of jasmonic acid hormones. It plays an important role in regulating plant defense responses, secondary metab olism and g rowth and d evelop ment p rocesses. Based on Salvia miltiorrhiza Bung e transcrip tomes and g enome survey seq uences,the MYC2 gene transcription factor in S.miltiorrhiza was cloned and named Sm MYC2（ Gen Bank accession number： KJ945636）. The c DNA sequence length of Sm MYC2 was 1910 bp with no introns,and the open reading frame（ ORF） was 1809 bp, encoding 602 amino acids. Moreover, Sm MYC2 contained no membrane-spanning domains or signal peptides, and had high homology with MYC2 of tobacco and tomato. Quantitative real-time PCR analysis showed that Sm MYC2 was expressedin the roots,stems,leaves and flowers of S. miltiorrhiza,with the highest levels of expression observed in the roots and stems. In addition,the expression of Sm MYC2 could be induced by methyl jasmonate,light and wounding,but could be repressed by gibberellin. The present study will be helpful for further functional research of Sm MYC2 in S. miltiorrhiza.

关 键 词：丹参 MYC2 BHLH 表达模式分析 

分 类 号：Q78[生物学—分子生物学]