和胃安神方对失眠模型大鼠血浆及下丘脑瘦素的影响  

Effect of Hewei Anshen Decoction on Leptin Levels in Plasma and Hypothalamus of Insomnic Rats

作　　者：黎发根[1] 李绍旦[1] 刘毅[1] 于磊[1] 杨明会[1] 

机构地区：[1]解放军总医院中医研究所,北京100853

出　　处：《中华中医药学刊》2016年第5期1084-1086,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81173429);首都医学发展基金项目(SF-2009-Ⅱ-16)

摘　　要：目的:探讨和胃安神方对失眠大鼠血浆及下丘脑瘦素(leptin)的影响。方法:将30只雄性Wistar大鼠随机分为正常对照组、失眠模型组、和胃安神方中药组。采用用放射免疫分析法检测血浆及下丘脑组织leptin的含量。结果:和胃安神方组血浆leptin含量为(1.405±0.347)ng/m L,低于模型组(2.159±0.421)ng/m L,差异具有统计学意义(P<0.01),与正常组(1.279±0.535)ng/m L比较无显著性差异(P>0.05);和胃安神方组下丘脑leptin含量为(55.094±9.991)pg/mg,低于模型组(74.877±10.977)pg/mg,差异具有统计学意义(P<0.01),与正常组(51.459±10.623)pg/mg比较无显著性差异(P>0.05)。结论:和胃安神方治疗失眠症的疗效机制可能与其调节leptin含量有关。Objective： To study the effect of Hewei Anshen Decoction（ HWASD） on leptin levels in plasma and hypothalamus of insomnic rats. Method： Thirty healthy male Wistar rats were randomly divided into three groups： normal control group,insomnia model group and HWASD treatment group. Radioimmunoassay was used to measure the levels of leptin in plasma and hypothalamus. Result： The concentration of leptin in the plasma of HWASD treatment group was（ 1. 405± 0. 347） ng/m L,significantly lower than that of insomnia model group[（ 2. 159 ± 0. 421） ng/m L]（ P〈0. 01） and similar with normal control group [（ 1. 279 ± 0. 535） ng/m L]（ P〉0. 05）. The levels of leptin in the hypothalamus of HWASD treatment group was（ 55. 094 ± 9. 991） pg/mg,significantly lower than that of insomnia model group[（ 74. 877± 10. 977） pg/mg]（ P〈0. 01） and similar with normal control group[（ 51. 459 ± 10. 623） pg/mg]（ P〉0. 05）. Conclusion： The therapeutic effect of Hewei Anshen Decoction on insomnia is partially attributed to the regulation of leptin levels.

关 键 词：和胃安神方 失眠 血浆 下丘脑 瘦素 

分 类 号：R285.5[医药卫生—中药学]