白藜芦醇对小鼠肾纤维化TGF-β_1/Smads信号转导通路的干预研究  

Resveratrol's Intervention on Renal Interstitial Fibrosis through TGF-β_1/Smads Signal Transduction Pathway in Mice

作　　者：陈薪薪[1] 仝欢[1] 陈宇[2] 吕吟秋[1] 李凡凡[1] 陈孝倩[1] 陈朝生[1] 

机构地区：[1]温州医科大学附属第一医院,浙江温州325000 [2]温州市中医院,浙江温州325000

出　　处：《中华中医药学刊》2016年第5期1224-1227,I0013,I0014,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：温州市科技计划项目(Y20120134)

摘　　要：目的:观察白藜芦醇对单侧输尿管梗阻(UUO)构建的小鼠肾纤维化模型TGF-β_1/Smads信号转导通路的影响,探讨其对肾纤维化的干预作用及其机制。方法:40只雄性ICR小鼠随机分为5组:假手术(Sham)组,UUO模型(UUO)组,UUO+白藜芦醇低剂量(20 mg/kg)治疗(ResL)组,UUO+白藜芦醇高剂量(40 mg/kg)治疗(ResH)组,UUO+氯沙坦(10 mg/kg)治疗(Los)组,每组8只。均于术后当天灌胃给药,每24 h 1次。14 d时处死小鼠,留取血清检测血肌酐(Scr)、尿素氮(BUN),肾组织行HE和Masson染色,观察病理结果,实时荧光定量PCR检测肾组织TGF-β_1、Smad2、Smad7 mRNA的表达。结果:白藜芦醇可以减轻肾组织纤维化的病理损害,使肾组织TGF-β_1、Smad2 mRNA表达降低,Smad7 mRNA表达升高(P<0.05,部分P<0.01),以高剂量组改善最显著。结论:白藜芦醇可呈剂量相关性下调TGF-β_1、Smad2及上调Smad7来抑制TGF-β_1/Smads信号转导通路,从而发挥抗肾间质纤维化的作用。Objective： To explore the mechanism of Resveratrol on renal interstitial fibrosis in UUO model mice and the TGF- β_1/Smads signal transduction pathway. Methods： Forty male ICR mice were randomly divided into five groups： the sham group（ Sham）,UUO model group（ UUO）,UUO ＋ low dose（ 20 mg/kg） Resveratrol group（ ResL）,UUO ＋ high dose（ 40 mg/kg） Resveratrol group（ ResH） and UUO ＋ Losatan（ 10 mg/kg） group（ Los）. Each group had eight mice. After operation,drugs were given intragastric administration once a day for consecutive 2 weeks. Mice were executed on the 14 th day. Scr and BUN in the blood serum were tested. The HE and Masson staining ways were applied to the kidney tissues.The renal mRNA of TGF- β_1,Smad2 and Smad7 were detected by real- time PCR. Results： The general condition of mice could be improved by Resveratrol. Pathological damage of kidney could be relieved. The expressions of TGF- β_1and Smad2 mRNA were significantly reduced while Smad7 mRNA could be increased（ P〈0. 05,partly P〈0. 01）. The most significance was in the high dose Resveratrol group（ ResH）. Conclusion： Resveratrol could inhibit TGF- β_1/Smads signal transduction pathway by down regulation of TGF- β_1and Smad2 and up regulation of Smad7 according to the dose and plays a protective effect on renal interstitial fibrosis.

关 键 词：白藜芦醇 肾纤维化 TGF-β1/Smads UUO 

分 类 号：R285.5[医药卫生—中药学]