氯吡脲(CPPU)处理对采后杧果保鲜及生理效应的影响  

Physiological effect of CPPU on delaying senescing of mango fruit during storage

作　　者：张鲁斌[1] 宋康华[1] 贾志伟 谷会[1] 弓德强[1] 

机构地区：[1]中国热带农业科学院南亚热带作物研究所.海南省热带园艺产品采后生理与保鲜重点实验室,广东湛江524091

出　　处：《果树学报》2016年第5期604-611,共8页Journal of Fruit Science

基　　金：中央级公益性科研院所基本科研业务费专项(1251022012001;1630062015012);海南省重大科技项目(ZDZX2013011);海南省自然科学基金(No.314111)

摘　　要：【目的】探讨氯吡脲[(1-(2-chloropyridin-4-yl)3-phenylurea,CPPU]处理对采后杧果(Mangifera indica L.)贮藏保鲜的影响,为杧果贮藏保鲜措施提供理论依据。【方法】以‘红芒6号’杧果为试验材料,采后经25 mg·L-1的CPPU浸果处理5 min后,在25℃条件下贮藏,采用气相色谱定期测定乙烯释放量,并采用常规理化分析方法测定杧果品质及相关生理指标。【结果】外源CPPU处理杧果,可以有效降低杧果果实在贮藏期间的病情指数,延缓可滴定酸(titratable acid,TA)、维生素C含量的下降,在贮藏15 d后,处理果实的病情指数比对照果实低20.99%,TA和维生素C含量分别比对照高出73.37%、30.42%;同时,在贮藏过程中,CPPU处理推迟杧果呼吸乙烯高峰2 d、延缓了PG(polygalacturonase)和Cx(cellulase)2个酶活性高峰3 d;同时,CPPU处理延缓了果实POD(peroxidase)和CAT(catalase)酶活性峰值的到来。【结论】外源CPPU处理杧果可以有效延缓采后杧果的衰老进程,是采后杧果贮藏保鲜的一种有效途径。【Objective】Mango is a typical climacteric fruit, belonging to the genus Mangifera in the family Anacardiaceae. Fruits are very difficult to store owing to quickly softening, rotting after the ethylene climacteric peak and losing their commercial value. So far, storage methods for mango include chemical,physical and biological means. Some drawbacks in physical preservation are higher equipment requirements, great investment and complex operation. Chemical preservation mainly uses fungicide, which might cause residue problem. So, more attention is paid on biological preservation. It is very important to find a safe and efficient preservation means for mango fruits.【Methods】The tested variety was‘Hongmangliuhao＇mango. Harvested mature fruits were transported to the lab immediately, the healthy fruits with same size were selected and were washed and dried in natural air ventilation for test. The fruits were dipped in 25 mg·L^-1CPPU [1-（2-chloropyridin-4-yl） 3-phenylurea, CPPU] solution for 5 min and dried at room temperature（25 ℃）, and ten dried fruits were placed in a plastic box which was put into polyethylene plastic bag and stored at room temperature. The fruits dipped in water were used as the control. The external changes and disease index of fruits were observed periodically. Physiological Indexes related to fruit quality were evaluated, such as fruit firmness, total soluble solids（TSS）, titratable acid（TA）, vitamin C content and ethylene production, and so on. Activities of fruit softening related enzymes were also measured e.g. polygalacturonase（PG） and cellulose（Cx）. The activities of catalase（CAT） and peroxidase（POD） were detected by spectrophotometer as well. In the experiments, three replicates were conducted with 30 fruits in each repeat. Statistical analysis of data was performed by Excel software. Significant differences were analyzed by SPSS software at the level of 0.05.【Results】The disease fruits appeared in mango fruits treated by CPPU at the 6th

关 键 词：杧果 CPPU 衰老 生理效应 

分 类 号：S667.7[农业科学—果树学]