不同施肥处理对甜菊生长及糖苷含量和积累量的影响  

Effects of different fertilizer treatments on growth,and content and accumulation of glycoside in Stevia rebaudiana

作　　者：包亚英[1] 胡秀英[2] 郝雨杉[1] 杨永恒[1] 原海燕[1] 黄苏珍[1] 

机构地区：[1]江苏省中国科学院植物研究所(南京中山植物园),江苏南京210014 [2]南京农业大学,江苏南京210095

出　　处：《植物资源与环境学报》2016年第1期71-80,共10页Journal of Plant Resources and Environment

基　　金：江苏省农业科技自主创新资金项目〔CX(13)2020〕;江苏省科技支撑计划项目(BE2014402)

摘　　要：采用盆栽法,以甜菊（Stevia rebaudiana Bertoni）品种‘中山4号’（‘Zhongshan No.4’）当年生扦插苗为研究对象,研究不同形态氮肥（硫酸铵、硝酸钠和尿素）及不同施氮量（纯氮）、施磷量（P2O5）和施钾量（K2O）对幼苗生长及糖苷含量和单株积累量的影响。结果显示：随氮肥、磷肥和钾肥施用量的提高,甜菊幼苗的株高、茎粗、叶长、叶宽、单株叶干质量和单株茎干质量均呈先升后降的变化趋势,且总体上与对照无显著差异,仅施磷量300 mg·kg^-1处理组的叶长显著高于对照;根据施肥量与单株叶干质量的回归方程,确定硫酸铵、硝酸钠、尿素、磷肥和钾肥的施用量分别为64.87、660.21、735.84、211.54和775.92 mg·kg^-1时,幼苗单株叶干质量最高。在硫酸铵处理组中,300 mg·kg^-1处理组甜菊叶片中的莱鲍迪苷A（R-A）含量及甜菊苷（St）、R-A和总苷的单株积累量以及600 mg·kg^-1处理组的St单株积累量高于对照,多数处理组的St、R-A和总苷含量及单株积累量均低于对照;在硝酸钠处理组中,1 200 mg·kg^-1处理组的R-A和总苷含量、600和900 mg·kg^-1处理组的St单株积累量以及300-900mg·kg^-1处理组的R-A和总苷单株积累量高于对照,其他处理组的St、R-A和总苷含量及单株积累量均低于对照;在尿素处理组中,1 500 mg·kg^-1处理组的R-A和总苷的含量和单株积累量以及600和900 mg·kg^-1处理组的R-A和总苷单株积累量高于对照,其他处理组的St、R-A和总苷含量及单株积累量均低于对照;各施氮处理组中,仅1 500 mg·kg^-1处理组的R-A含量与对照差异显著,其他指标均与对照无显著差异。在施磷处理组中,100 mg·kg^-1处理组的R-A含量以及100和200 mg·kg^-1处理组的St、R-A和总苷的单株积累量高于对照,多数处理组的St、R-A和总苷含量及单株积累量低于对照且与对照均无显著差异。在施钾处理组中,各处理组的St、R-A和总苷�Taking annual cutting seedlings of cultivar‘Zhongshan No. 4＇of Stevia rebaudiana Bertoni as research objects,effects of different forms of nitrogen fertilizer 〔（ NH4）2SO4,Na NO_3 and CO（ NH_2）_2〕and different applying amounts of nitrogen（ pure nitrogen）,phosphate（ P2O5） and potassium（ K2O）fertilizers on seedling growth,and content and accumulation per plant of glycoside were studied by pot method. The results show that with enhancing of applying amounts of nitrogen,phosphate and potassium fertilizers,height,stem diameter,leaf length,leaf width,leaf dry weight per plant and stem dry weight per plant of seedling all appear the trend of firstly increasing and then decreasing,and generally there is no significant difference with the control,only leaf length in the treatment group with applying amount of phosphate fertilizer of 300 mg · kg- 1is significantly higher than that of the control. According to regression equation between applying fertilizer amount and leaf dry weight per plant,when applying amounts of（ NH_4）_2SO_4,Na NO_3,CO（ NH2）2,phosphate and potassium fertilizers are 64. 87,660. 21,735. 84,211. 54 and 775. 92 mg · kg- 1,respectively,leaf dry weight per plant of seedling is the highest. In（ NH_4）_2SO_4treatment group,content of rebaudioside A（ R-A） in leaf and accumulations per plant of stevioside（ St）,R-A and total glycosides of S. rebaudiana in the treatment group with 300mg·kg- 1and accumulation per plant of St in the treatment group with 600 mg ·kg- 1are higher than those of the control,contents and accumulations per plant of St,R-A and total glycosides in most treatment groups are lower than those of the control. In Na NO_3 treatment group,contents of R-A and total glycosides in the treatment group with 1 200 mg ·kg- 1,accumulation per plant of St in the treatment groups with 600 and 900 mg · kg- 1and accumulations per plant of R-A and total glycosides in the treatment groups with 300- 900 mg · kg- 1are higher than those of the control,and conten

关 键 词：甜菊 氮磷钾肥 氮素形态 生长指标 糖苷含量 糖苷积累量 

分 类 号：S512.1[农业科学—作物学] S606.2