绵羊李斯特菌在小鼠体内的感染增殖规律及诱导的细胞因子分泌  

Infection and proliferation mechanism and cytokine production in Listeria ivanovii infected mice

作　　者：周梦莹[1] 蒋明娟[1] 任晨艳 刘思静[1] 蒲启康 汪川[1] 

机构地区：[1]四川大学华西公共卫生学院(华西第四医院),四川成都610041

出　　处：《现代预防医学》2016年第9期1644-1648,共5页Modern Preventive Medicine

基　　金：成都市科技局科技惠民技术研发项目;李斯特菌活菌载体疫苗构建通用平台及其在结合疫苗研究中的应用;2014-HM01-00370-SF;国家自然科学基金;李斯特菌载体多阶段结核疫苗体系的构建及其诱导免疫应答与效果的研究;31570924

摘　　要：目的研究绵羊李斯特菌（Li）静脉接种和滴鼻接种C57BL／6J小鼠后，细菌在小鼠体内感染增殖规律及诱导的细胞免疫应答水平，为Li作为一种新型疫苗载体提供科学依据。方法采用寇氏法测定Li静脉接种和滴鼻接种C57BL／6J小鼠的半数致死剂量（LD50）；以0．1×LD50剂量静脉或滴鼻接种小鼠，测定不同时间点小鼠肝、脾和肺中的细菌载量、组织病理学改变，以及肝和脾组织匀浆液中IFN-γ肺组织匀浆液中IFN-γ、TNF-α和IL-6的含量。结果Li静脉接种和滴鼻接种小鼠的半数致死剂量分别为6．3×106（cfu）和2．5×108（cfu）。Li静脉感染小鼠后能够在肝、脾和肺中增殖，引起组织细胞坏死和炎性浸润并诱导IFN-γ泌水平上调，三种器官中肝的各指标变化最显著。“滴鼻感染小鼠后主要在肺部增殖，引起肺部病理损害并诱导肺组织IFN-γ、TNF-α和IL-6分泌水平上调。结论静脉接种Li可以在小鼠体内引起全身性多器官的病理和免疫反应，而滴鼻接种Li可以针对性地在小鼠肺部引起病理和免疫反应；Li可以作为一种新型疫苗载体。Objective To study the infection and proliferation mechanism and levels of cytokines production in Listeria ivanovii （Li） intravenously or intranasally infected C57BL/6J mice, and to provide scientific basis for using Li as a novel vaccine vector. Methods The median lethal doses of Li for intravenous or intranasal infection of C57BL/6J mice were determined by Kaeber method. Then mice were infected intravenously or intranasally with Li at the doses of 0. 1 median lethal, bacterial loads and histopathologic changes in the liver,spleen and lung at indicated time points post infection after each inoculation were determined and analyzed,and IFN -γ/ titers in liver homogenate and spleen homogenate,as well as IFN -γ,TNF -α and IL- 6 titers in lung homogenate were determined. Results Median lethal dose of Li for intravenous inoculation and intranasal inoculation were 6.3×106 cfu and 2.5 ×108 cfu respectively. After intravenous inoculation,Li could proliferate in liver,spleen and lung, causing cell destruction and inflammatory infiltration and lead to promoted IFN - γ production, especially in the liver. After iutranasal inoculation, Li proliferated mostly in the lung, causing lung tissue damages and promoted production of IFN -γ, TNF -αand IL - 6, Conclusion Intravenous inoculation of Li could induce multi - organs pathogenic and immune reactions in mice while intranasal inoculation of Li could only induce notable pathogenic and immune reactions in the lung; Li could be considered as a novel vaccine vector.

关 键 词：绵羊李斯特菌 静脉接种 滴鼻接种 感染规律 细胞因子 

分 类 号：R113[医药卫生—公共卫生与预防医学]