右归饮对兔膝骨关节炎软骨细胞凋亡中Fas/FasL蛋白表达的影响  

Effect of Youguiyin on the expression of Fas/FasL protein in the apoptosis of cartilage cells of rabbit knee osteoarthritis

作　　者：武中庆[1] 邓闵军 郭攀[1] 王丹[1] 袁永健[1] 闵继康[1] 徐旭纯[1] 段佳忠[1] 钟建明[1] 李恒[1] 

机构地区：[1]浙江省湖州市第一人民医院,湖州313000

出　　处：《浙江创伤外科》2016年第2期202-205,共4页Zhejiang Journal of Traumatic Surgery

基　　金：湖州市科技局公益性技术应用研究项目(编号:2013GY26)

摘　　要：目的探讨右归饮对兔膝骨关节炎软骨细胞凋亡中Fas/Fas L蛋白表达的影响,分析中药右归饮对膝骨关节炎软骨细胞的凋亡干预作用、干预途径。方法建立兔膝骨关节炎动物模型,将选取的48只实验兔随机分为正常组、模型组、实验组与阳性对照组,各组各12只;正常组、模型组给予标准饲料喂养,实验组予右归饮灌胃,阳性对照组予美洛昔康灌胃。处死后行体外分离培养兔膝骨关节炎软骨细胞,运用TUNEL法测定凋亡细胞,另观察病理形态,并通过免疫组化学法测定各组Fas/Fas L蛋白表达。结果实验组、阳性对照组细胞凋亡指数显著高于正常组,显著低于模型组,实验组细胞凋亡指数显著高于阳性对照组(P<0.05);正常组病理图片中有少量Fas/Fas L蛋白表达,模型组Fas/Fas L蛋白表达显著,阳性对照组Fas/Fas L蛋白表达较模型组有所减少,但较实验组多;实验组、阳性对照组Fas蛋白、Fas L蛋白表达显著高于正常组,实验组Fas蛋白、Fas L蛋白表达显著低于模型组(P<0.05);阳性对照组Fas蛋白、Fas L蛋白表达与模型组无显著差异,实验组Fas蛋白、Fas L蛋白表达与阳性对照组无显著差异(P>0.05)。结论中药右归饮有助于抑制Fas/Fas L蛋白表达,从而实现对膝骨关节炎软骨细胞凋亡的抑制,对膝骨关节炎软骨细胞有一定的保护作用。Objective To study the effect of on the expression of Fas/FasL in the apoptosis of cartilage cells of rabbit knee osteoarthritis, and to analyze the effect of traditional Chinese medicine decoction on the apoptosis of cartilage cells in the knee osteoarthritis. Methods to establish the rabbit knee osteoarthritis animal model, 48 rabbits were randomly divided into normal group, model group, experimental group and positive control group, 12 in each group. In the normal group, model group the standard diet were given. In the experimental group, the rabbits were received Youguiyin gavage, and meloxicam orally was given in the positive control group. The cartilage cells of rabbit knee osteoarthritis were isolated and cultured in vitro. The TUNEL method was used to determine the apoptotic cells, and the pathological changes were observed. The expression of Fas/FasL protein was detected by immunohistochemical method. Results In the experimental and positive control groups, the apoptosis index was significantly higher than that in the normal group, and significantly lower than that in the model group, the apoptosis index in the model group was significantly higher than that in the positive control group（P〈0.05）. in the normal group, pathological picture had little Fas/Fas L expression, in the model group was significantly expressed. In the positive control group, the expression of Fas/Fas L protein was reduced compared to the model group,but higher than that in the experimental group. In the experimental group and positive control group the expression of Fas protein and Fas L protein were significantly higher than that in the normal group, the expression of Fas protein and Fas L protein in experimental group was significantly lower than that in model group（P〈0.05）. The expression of Fas protein and Fas L protein in the positive control group had no significant difference than that in the model group, and there was no significant difference between the positive control group and the experimental group（ P〉

关 键 词：右归饮 膝骨关节炎 软骨细胞 细胞凋亡 FAS/FASL蛋白 

分 类 号：R687.3[医药卫生—骨科学]