Cloning of four DREB genes from Tibetan Sophora moorcroftiana and analysis of their expression during abiotic stress  

作　　者：Weijie Yao Yaru Fu Yanfu Zhang Hui-e Li 

机构地区：[1]Agricultural and Animal Husbandry College, Tibet University, Linzhi 860000, Tibet, People's Republic of China [2]Key Laboratory of Forest Ecology in Tibet Plateau (Tibet University), Ministry of Education, Linzhi 860000, Tibet,People's Republic of China [3]National Key Station for Field Scientific Observation &Experiment, Linzhi 860000, Tibet, People's Republic of China

出　　处：《Journal of Forestry Research》2016年第3期675-683,共9页林业研究（英文版）

基　　金：supported by the National Natural Science Foundation of China(31260189);Collaborative Innovation Center Construction of Research and Development on Tibetan Characteristic Agricultural and Animal Husbandry Resources on plateau ecology;Animal Husbandry Resources and Promotion Plan of Plateau Basic Ecological Academic Team Ability,Agricultural and Animal Husbandry College,Tibet University

摘　　要：Sophora moorcroftiana is an endemic, droughtresistant shrub that grows in Tibet and has some degree of resistance to salt, cold, heat, and drought. In the present study, four dehydration responsive element-binding（DREB） genes（Sm DREB1, Sm DREB2, Sm DREB and Sm DREB1） were isolated from S. moorcroftiana for the first time and their expression and proline content under abiotic stress were analyzed. Proline accumulated in seedlings under drought, salt, cold, and heat stress treatments. The four genes were variously expressed in response to the four abiotic stresses. Sm DREB1 was induced by drought, cold, and heat stresses; Sm DREB2 and Sm DREB4 were both induced by salt, cold, and heat stresses, whereas Sm DREB3 was induced by drought and heat stresses. Thus, these four genes may participate in conferring tolerance to these four abiotic stresses and are candidate genes for genetic engineering in the future.Sophora moorcroftiana is an endemic, droughtresistant shrub that grows in Tibet and has some degree of resistance to salt, cold, heat, and drought. In the present study, four dehydration responsive element-binding（DREB） genes（Sm DREB1, Sm DREB2, Sm DREB and Sm DREB1） were isolated from S. moorcroftiana for the first time and their expression and proline content under abiotic stress were analyzed. Proline accumulated in seedlings under drought, salt, cold, and heat stress treatments. The four genes were variously expressed in response to the four abiotic stresses. Sm DREB1 was induced by drought, cold, and heat stresses; Sm DREB2 and Sm DREB4 were both induced by salt, cold, and heat stresses, whereas Sm DREB3 was induced by drought and heat stresses. Thus, these four genes may participate in conferring tolerance to these four abiotic stresses and are candidate genes for genetic engineering in the future.

关 键 词：Proline content Expression profile Droughtstress Salt stress - Cold stress Heat stress 

分 类 号：S793.9[农业科学—林木遗传育种]