人脐带间充质干细胞分泌白介素-6对白血病细胞分化的影响  

Human Umbilical Cord-derived Mesenchymal Stem Cells Secrete Interleukin-6 to Influence Differentiation of Leukemic Cells

作　　者：陈芳[1] 马凤霞[1] 李洋[1] 徐方运[1] 池颖[1] 卢士红[1] 韩忠朝[1] 

机构地区：[1]中国医学科学院北京协和医学院血液学研究所血液病医院实验血液学国家重点实验室,天津300020

出　　处：《中国医学科学院学报》2016年第2期164-168,共5页Acta Academiae Medicinae Sinicae

基　　金：国家重点基础研究发展计划(973计划)(2011CB964802);国家自然科学基金(81500098);国家科技支撑计划(2013BAI01B09)~~

摘　　要：目的探讨脐带来源的间充质干细胞(UC-MSC)对白血病细胞分化的影响。方法体外建立UC-MSC与急性早幼粒细胞白血病细胞系NB4细胞共培养体系,通过细胞形态、硝基四唑氮蓝还原实验和细胞表面粒系分化标志CD11b的检测,评价肿瘤细胞的分化状态。结果 UC-MSC在体外能够诱导NB4细胞向粒系分化成熟并和小剂量全反式维甲酸共同应用时存在诱导作用联合增强。白介素(IL)-6Ra中和阻断IL-6作用后可部分逆转UC-MSC的促分化作用,外源性IL-6对NB4细胞发挥促粒系分化作用。结论 UC-MSC通过分泌IL-6可以诱导急性早幼粒细胞白血病细胞向粒系分化成熟,且和小剂量全反式维甲酸同时应用可以达到诱导分化作用联合增强。Objective To investigate the effect of human umbilical cord-derived mesenchymal stem cells（ UC-MSC） on the differentiation of leukemic cells. Method The co-culture system of UC-MSC with acute promyelocytic leukemic cell line NB4 cells was constructed in vitro,and the differentiation status of the leukemic cells was assessed by cell morphology,nitroblue tetrazolium reduction test,and cell surface differentiation marker CD11 b. Results UC-MSC induced the granulocytic differentiation of NB4 cells. When UC-MSC and a small dose of all-trans retinoic acid were applied together,the differentiation-inducing effect was enhanced in an additive manner. Interleukin（ IL）-6Ra neutralization attenuated differentiation and exogenous IL-6-induced differentiation of leukemic cells. Conclusion UC-MSC can promotd granulocytic differentiation of acute promyelocytic leukemia cells by way of IL-6 and presented additive effect when combined with a small dose of all-trans retinoic acid.

关 键 词：间充质干细胞 急性早幼粒细胞白血病 NB4 分化 白介素-6 

分 类 号：R557.2[医药卫生—血液循环系统疾病]