小鼠睾丸中细胞连接相关蛋白质的分析  

Expression Patterns of the Proteins Associated with Cell Junctions in Mouse Testes

作　　者：付俊[1] 罗艳云[1] 缪时英[1] 王琳芳[1] 

机构地区：[1]中国医学科学院基础医学研究所北京协和医学院基础学院医学分子生物学国家重点实验室生物化学与分子生物学系,北京100005

出　　处：《中国医学科学院学报》2016年第2期205-209,共5页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金青年科学基金(31401273);国家重大科学研究计划(2012CB944902)~~

摘　　要：目的探讨细胞连接相关蛋白在不同发育阶段小鼠睾丸中的表达模式。方法 Western blot检测并分析1~6周龄小鼠睾丸、原代支持细胞以及小鼠精原细胞系GC1 spg、精母细胞系GC2 spg、间质细胞系TM3、支持细胞系TM4等4种生殖相关细胞系中细胞连接相关蛋白的表达水平。结果小鼠睾丸曲细精管中支持细胞间细胞连接相关膜蛋白连接黏附分子A、紧密连接蛋白Occludin和Claudin,以及支持细胞-生精细胞间细胞连接相关膜蛋白连接黏附分子C、连接蛋白Nectin-3和钙黏附蛋白E的表达表现出明显的阶段特异性。而参与细胞连接的调节蛋白紧密连接蛋白ZO-1、ZO-2、Afadin、β链蛋白和CD2相关蛋白在睾丸的不同发育阶段以及生殖细胞系中表达无差异。结论小鼠睾丸曲细精管中支持细胞间以及支持细胞-生精细胞间细胞连接相关的膜蛋白多表现出明显的阶段特异性。而参与细胞连接的调节蛋白在睾丸的不同发育阶段中表达无差异。Objective To study on the expression patterns of proteins associated with cell junctions in the developing mouse testes. Method The expression levels of reproductive related cell lines spermatogonia cell line GC1 spg,spermatocyte cell line GC2 spg,leydig cell line TM3,and sertoli cell line TM4,primary sertoli cells,and 1-6-week mouse testes were analyzed using Western blot. Results The sertoli cell junction-associated membrane proteins adhesion molecule A,Occludin and Claudin,and the sertoli-germ cell junction-associated membrane proteins junctional adhesion molecule C,Nectin-3,and E-cadherin were stage-specific in the seminiferous tubules in the mouse testes. The adaptor proteins associated with cell juctions zonula occludens-1,zonula occludens-2,Afadin,β-catenin,and CD2-associated protein were not stage-specific in the seminiferous tubulesin the mouse testes. Conclusions In the seminiferous tubules in the mouse testes,the membrane proteins associated with cell junctions are stage-specific. However,the expressions of adaptor proteins associated with cell junctions do not obviously change.

关 键 词：血睾屏障 支持细胞间连接 支持细胞-生精细胞间连接 膜蛋白 调节蛋白 

分 类 号：Q249[生物学—细胞生物学]