雷公藤多苷对糖尿病大鼠肾脏的保护作用  

Protective effects of tripterygium wilfordii polyglycosides on kidney of diabetic rats

作　　者：厉莉[1] 赵然[3] 李春君[2] 孙蓓[1] 杨薇[1] 马泽军[1] 张晓娜[1] 郭欣[1] 陈莉明[1] 

机构地区：[1]天津医科大学代谢病医院糖尿病肾病科,卫生部激素与发育重点实验室,300070 [2]天津医科大学代谢病医院糖尿病谢病科,300070 [3]北京电力医院内分泌科

出　　处：《中国糖尿病杂志》2016年第5期459-464,共6页Chinese Journal of Diabetes

基　　金：国家自然科学基金(81470187);天津市应用基础与前沿技术研究计划(14JCYBJC26200)

摘　　要：目的探讨不同剂量雷公藤多苷(TWP)对糖尿病大鼠肾脏的保护作用。方法高糖高脂喂养联合小剂量STZ(30 mg/kg)构建T2DM大鼠模型,予不同剂量TWP治疗8周,测定大鼠24 hUAlb及血液生化指标,并观察肾脏病理变化,检测肾脏nephrin、podocin和血管内皮生长因子(VEGF)表达水平的变化。结果 DM组24 hUAlb高于NC组[(253.7±53.0)vs(45.7±14.5)μg/24 h];与DM组比较,TWP干预后,24 hUAlb减少[(253.7±53.0)vs(183.5±78.7)、(123.8±74.8)、(119.0±52.0)μg/24 h],TWP可改善糖尿病大鼠肾脏病理改变,并使nephrin和podocin表达量增高,VEGF表达量下降。结论 TWP通过上调nephrin和podocin及下调VEGF的表达,对糖尿病大鼠肾脏起到保护作用。Objective To explore the protective effects of different doses of tripterygium wilfordii polyglycosides（TWP） on kidney of diabetes melltus（DM） rats.Methods T2 DM rats were induced by8-week high-sugar and high-fat diets followed injection of 30 mg/kg streptozotocin（STZ）.After 8 weeks TWP treatment,levels of 24 h urine albumin（24 hUAlb） and blood biochemical parameters were measured.Pathological morphology of the kidney was observed by hematoxylin-eosin（HE） and Masson stains.The expression of nephrin,podocin and vascular endothelial growth factor（VEGF） were measured by qPCR,western blot and immunohistochemical staining.Results Compared with the normal control（NO group,24 hUAlb was elevated in the DM group[（253.7 ± 53.0） vs（45.7±14.5） μg/24 h]（P〈0.05）.However,compared with DM group,24 hUAlb was decreased in a dose dependent manner after TWP treatment[（253.7±53.0） vs（183.5±78.7）,（123.8±74.8）,（119.0±52.0） μg/24 h].There were no significant differences in blood glucose before and after TWP treatment.Pathological examination showed that the changes of kidney in the DM group were improved after the administration of TWP.Interestingly,compared with the DM group,the levels of nephrin and podocin were markedly increased and VEGF was decreased following TWP treatment,especially in the middle and high doses of TWP.Conclusion TWP can up-regulate podocin and nephrin expression and down-regulate VEGF expression to protect kidney in diabetic rats.

关 键 词：糖尿病肾病 雷公藤多苷 NEPHRIN PODOCIN 血管内皮生长因子 

分 类 号：R285.5[医药卫生—中药学]