盐酸氨基葡萄糖对关节内出血引起的软骨损伤修复作用研究  

PROTECTIVE EFFECT OF GLUCOSAMINE-HYDROCHLORIDE ON CARTILAGE IN BLOOD-INDUCED JOINT DAMAGE IN VIVO

作　　者：张铭华[1] 张施洋[2] 赵增辉[2] 李维朝[2] 蒋电明[2] 

机构地区：[1]重庆医科大学附属永川医院骨科,重庆402160 [2]重庆医科大学附属第一医院骨科

出　　处：《中国修复重建外科杂志》2016年第5期562-568,共7页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：重庆市教委科学技术研究项目(KJ110327);重庆市医学科研计划项目(2013-1-011)~~

摘　　要：目的探讨盐酸氨基葡萄糖(glucosamine-hydrochloride,Glu/Ch)对关节内出血引起的软骨损伤(blood-induced joint damage,BJD)修复作用。方法取健康成年新西兰大白兔32只,随机分为高剂量Glu/Ch组(A组)、低剂量Glu/Ch组(B组)、阳性对照组(C组)、阴性对照组(D组),每组8只。A、B、C组制备关节内出血模型,D组不建模。建模后第1天开始A、B组每天以Glu/Ch灌胃(剂量分别为250、21.5 mg/kg)8周,C、D组以生理盐水灌胃8周。建模后3、7 d及2、8周检测关节损伤标志物血清软骨寡聚基质蛋白(cartilage oligomeric matrix protein,COMP)、血清硫酸软骨素846(chondroitin sulfate 846,CS846)、尿Ⅱ型胶原羧基端前肽(C-terminal telopepide of typeⅡcollagen,CTX-Ⅱ)浓度。8周时处死动物,抽取关节液采用ELISA法检测炎性因子IL-1β、TNF-α浓度。取关节软骨组织分别行免疫组织化学染色,测量基质金属蛋白酶13(matrix metalloproteinase 13,MMP-13)表达量;行阿尔新蓝、番红O染色,计算阳性染色面积百分比;对关节软骨中的蛋白多糖含量行半定量检测。结果建模后3 d A、B、C组COMP浓度显著高于D组(P<0.05),B、C组高于A组(P<0.05);7 d A、B、D组间比较差异无统计学意义(P>0.05),且均低于C组(P<0.05);2、8周各组间比较差异均无统计学意义(P>0.05)。各时间点各组间CS846浓度比较,差异均无统计学意义(P>0.05)。各时间点A、B、C组CTX-Ⅱ浓度均显著高于D组(P<0.05);除3 d外,7 d及2、8周时,A组CTX-Ⅱ浓度显著低于B、C组(P<0.05)。建模后8周,A、B组TNF-α浓度显著高于D组、低于C组(P<0.05);A、B组间比较差异无统计学意义(P>0.05)。A、B、D组IL-1β浓度显著低于C组(P<0.05),A、D组低于B组(P<0.05),A、D组间比较差异无统计学意义(P>0.05)。免疫组织化学染色示,C组MMP-13表达量显著高于A、B、D组,A、B组高于D组(P<0.05),A、B组间比较差异无统计学意义(P>0.05)。各组均见阿尔新蓝及番红O阳性染色,其中D组染色Objective To discuss the effect of glucosamine-hydrochloride （Glu/Ch） in protecting and repairing the cartilage in blood-induced joint damage （BJD） in vivo. Methods Thirty-two adult New Zealand rabbits were randomly divided into 4 groups （n--8）： high-dose Glu/Ch treated group （group A）, low-dose Glu/Ch treated group （group B）, positive control group （group C）, and negative control group （group D）. A joint bleeding model was established by blood injection into articular cavity in groups A, B, and C. Glu/Ch was given by gavage in groups A （250 mg/kg） and B （21.5 mg/kg） once a day for 8 weeks, and the same dosage of saline was given in groups C and D. The serum cartilage oligomeric matrix protein （COMP）, serum chondroitin sulfate 846 （CS846）, and urinary C-terminal telopepide of type II collagen （CTX-II） were measured at 3 days, 7 days, 2 weeks, and 8 weeks after modeling. The expressions of cytokines such as interleukin 113 （IL-1β） and tumor necrosis factor u （TNF-α） in synovial fluid were analyzed by ELISA at 8 weeks after modeling. The expression of matrix metalloproteinase 13 （MMP-13） was detected by immunohistochemistry. Alcian blue staining and Safranin-O staining were performed to calculate the percentage of the positive staining areas. The proteoglycan content was detected by semi-quantitative analysis in the articular cartilage. Results The COMP concentration was significantly higher in groups A, B, and C than group D, and in groups B and C than group A at 3 days after modeling （P〈0.05）; no significant difference was found among groups A, B, and D at 7 days （P〉0.05）, and it was significantly lower in groups A, B, and D than group C （P〈0.05）; there was no significant difference among 4 groups after 2 and 8 weeks （P〉0.05）. Difference in CS846 concentration had no significance among 4 groups at each time point （P〉0.05）. The CTX-Ⅱ concentration of groups A, B, and C was significantly higher than that of group D at ea

关 键 词：关节内出血 盐酸氨基葡萄糖 软骨修复 兔 

分 类 号：R684[医药卫生—骨科学]