携iRGD肽阳离子微泡的制备及其基本性质检测  

Preparation and Characterization of iRGD Targeted Cationic Microbubbles

作　　者：曾欣欣[1] 徐金锋[1] 刘莹莹[1] 周玉丽[1] 罗慧[1] 

机构地区：[1]暨南大学第二临床医学院暨深圳市人民医院超声科,深圳市518020

出　　处：《中国超声医学杂志》2016年第5期471-473,共3页Chinese Journal of Ultrasound in Medicine

基　　金：深圳市科技研发资金(No.JCYJ20130402092657773);广东省自然科学基金项目(No.2014A030313760)

摘　　要：目的将iRGD肽和PEI600连接至微泡上,制备靶向阳离子微泡。方法采用硫醇-马来酰亚胺法连接iRGD肽,通过PEI600修饰使微泡表面电荷呈现正电荷,观察制备的携iRGD肽阳离子微泡(MBiRGD)与bEnd.3内皮细胞和人MCF-7乳腺癌细胞的黏附情况,并检测MBiRGD结合质粒DNA的情况。结果制备的MBiRGD表面电荷为正电荷,能有效结合质粒DNA,并能靶向bEnd.3内皮细胞和人MCF-7乳腺癌细胞,其黏附能力明显高于非靶向微泡。结论制备的携iRGD肽阳离子微泡是一种能靶向肿瘤细胞的新型阳离子微泡。Objective Preparate targeting microbubbles which are coated with iRGD peptides and PEI600.Methods Use the thiol-maleimide method to conjugate iRGD peptides to the surface of microbubbles.To get positively charged microbubbles by PEI600 modifying.Observe the adhesion of the iRGD targeted cationic microbubbles（MBiRGD）ane bEnd.3cells and human breast cancer MCF-7cells.Detecting the ability to carry plasmid DNA of the MBiRGD.Results The surface charge of MBiRGDis positive.The microbubbles can targeted to bEnd.3cells and human breast cancer MCF-7cells,showed significantly higher adhesion than that of non-targeted microbubbles.ConclusionsThe iRGD targeted cationic microbubbles is a novel cationic microbubbles that can target to tumor cells.

关 键 词：靶向微泡 基因 肿瘤 

分 类 号：R943[医药卫生—药剂学]