HPLC法测定人血浆中胺碘酮及其代谢产物去乙基胺碘酮的浓度  

Determination of Amiodarone and Its Metabolite Desethylamiodarone in Human Plasma by HPLC

作　　者：沈雄荣[1] 宋向明[1] 叶勇[1] 杨水新[1] 

机构地区：[1]湖州市中心医院临床药学科,浙江湖州313000

出　　处：《中国药房》2016年第14期1919-1921,共3页China Pharmacy

摘　　要：目的：建立测定人血浆中胺碘酮及其代谢产物去乙基胺碘酮浓度的方法。方法：血浆采用pH 5.4的磷酸二氢钾缓冲液1ml酸化,以乙醚提取后进样,采用高效液相色谱法测定,色谱柱为Ultimate C18,流动相为醋酸-三乙胺缓冲溶液（A）-甲醇（B）（VA/VB=19∶81）,检测波长为242 nm,流速为1.0 ml/min,内标为黄体酮。结果：胺碘酮、去乙基胺碘酮血药浓度分别在0.2-4.0μg/ml（r=0.999 5）、0.24-4.8μg/ml（r=0.999 8）范围内线性关系良好,方法回收率分别为95.63%-100.10%、93.14%-106.70%（RSD≤9.7%）。结论：本方法方便快捷、准确,可操作性强,能快速准确检测出患者的胺碘酮及去乙基胺碘酮血药浓度。OBJECTIVE：To establish the method for the concentration determination of amiodarone and its metabolite desethylamidarone in plasma. METHODS：The plasma was acidized with pH 5.4 monobasic potassium phosphate and extracted with ethyl ether. HPLC method was adopted,and Ultimate C18 column was used with mobile phase consisted of glacial acetic acid-triethylamine buffer solution（A）-methanol（B）（VA/VB=19 ∶ 81）at the detection wavelength of 242 nm and flow rate of 1.0 ml/min using progestrone as internal standard. RESULTS：The linear range of amiodarone and desethylamiodarone were 0.2-4.0 μg/ml（r=0.999 5）and 0.24-4.8 μ g/ml（r=0.999 8）,and their recoveries were 95.63%-100.10% and 93.14%-106.70%（RSD≤9.7%）. CONCLUSIONS：The method is simple,accurate and practical,and it can quickly and accurately determinate the blood concentration of amiodarone and desethylamiddarone.

关 键 词：高效液相色谱法 胺碘酮 去乙基胺碘酮 血药浓度 

分 类 号：R969.1[医药卫生—药理学]