小鼠肾脏缺血再灌注期间乙醛脱氢酶2表达的变化  

Expression changes of aldehyde dehydrogenase 2 during renal ischemia-reperfusion in mice

作　　者：和安 梁茜子 袁清 洪善娟[3] 陈昌庆 李树欣 张大伟 蔡明 

机构地区：[1]解放军第三0九医院器官移植研究所,北京100091 [2]上海交通大学医学院附属第九人民医院北院病理科,上海302190 [3]清华大学免疫学研究所,北京100084

出　　处：《中华器官移植杂志》2016年第1期46-49,共4页Chinese Journal of Organ Transplantation

基　　金：国家自然科学基金（81200547,81470043）

摘　　要：目的探讨缺血再灌注（IR）期间小鼠肾组织乙醛脱氢酶2（ALDH-2）表达的变化。方法采用雄性C57BL／6J小鼠夹闭双侧肾蒂的方法建立小鼠肾脏IR损伤模型，肾蒂夹闭时间为30min。采用随机数字表法将小鼠分为7组：假手术组、缺血再灌注1h、3h、6h、12h、24h及72h组，每组8只小鼠。分别于假手术后1d及再灌注后1、3、6、12、24及72h获取各组小鼠肾脏，采用实时荧光定量逆转录聚合酶链反应（Real-timePCR）法检测肾组织ALDH-2mRNA的表达，采用蛋白质印迹法检测肾组织ALDH=2蛋白的表达。结果小鼠肾脏IR后肾组织ALDH-2mRNA的水平迅速升高，至再灌注后3h时达到峰值，与假手术组比较，IR3h组和IR6h组均显著升高（P〈0．001）；小鼠肾脏氓后肾组织ALDH-2蛋白表达逐渐降低，至再灌注后3h降至最低，之后逐渐升高，至再灌注后24h达到峰值，与假手术组比较，各瓜组肾组织ALDH-2蛋白表达的差异均有统计学意义（P〈0．01）。结论肾脏IR后ALDH-2在转录和蛋白水平的表达均有显著改变；IR过程对肾脏AL—DH-2的表达产生影响，并间接影响其对肾脏氓后致伤效应的调控作用。Objective To observe the expression change of aldehyde dehydrogenase 2 （ALDH- 2） in renal tissues of mice under ischemia-reperfusion （IR） injury. Method Male C57BL/6J mice IR injury model was establish by clipping bilateral renal pedicles for 30 min. Fifty-six mice were divided into 7 groups by using the random number table method： control group, and IR 1 h, 3 h, 6 h, 12 h, 24 h and 72 h groups. Kidneys in IR 1 h, 3 h, 6 h, 12 h, 24 h and 72 h groups were harvested at each time point, and those in the control group were harvested at 24 h of sham operation. Total mRNA expression levels of ALDH-2 in the kidneys were detected by real-time PCR. Western blotting was used to detect the ALDH-2 protein expression. Result The mRNA expression levels of ALDH-2 were increased right after IR and reached the peak at 3 h. The expression levels of ALDH-2 mRNA in IR 3 h group and IR 6 h group were significantly higher than in control group （P〈0. 001）. The expression of ALDH-2 protein began to decline after IR and reached a minimum at 3 h, then ALDH-2 protein expression was increased and reached its peak at 24 h （P〈0. 01）. The ALDH-2 expression in IR groups was significantly lower than in control group （ P 〈 0. 01 or P 〈 0. 001 ）. Conclusion The expression of ALDH-2 has a remarkable change after kidney IR, suggesting that the process of IR has an impact on ALDH-2 expression, and indirectly affects the function of regulation of ALDH-2 in kidney IR injury.

关 键 词：小鼠 肾脏 缺血再灌注 乙醛脱氢酶2 

分 类 号：R699.2[医药卫生—泌尿科学]