鳖甲煎丸诱导肝癌细胞凋亡及对STAT信号通路的影响  

Antitumor Effect of Biejiajian Pill on H22 Tumor Bearing Mice and Study on its Morphology

作　　者：孙阳[1] 吴勃岩[1] 车艳新 王雪[1] 徐放[1] 李明珠[1] 梁颖[1] 王艳杰[1] 姜德友[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《时珍国医国药》2016年第4期849-851,共3页Lishizhen Medicine and Materia Medica Research

基　　金：中国博士后科学基金资助项目(No.2014M551288);黑龙江省博士后资助项目(No.LBH-Z13205);黑龙江中医药大学优秀青年教师支持计划(No.051234)

摘　　要：目的通过观察鳖甲煎丸作用H22移植瘤后细胞形态学变化,探讨鳖甲煎丸体内抗肿瘤的作用机制。方法将小鼠肝癌细胞移植于ICR小鼠皮下,应用不同剂量鳖甲煎丸(0.411,1.233,3.699 g·kg-1)ig给药,环磷酰胺0.03 g·kg-1ip给药,测定各组抑瘤率和脾指数;采用HE染色光镜下观察各组形态的变化;应用透射电镜观察模型组和鳖甲煎丸组细胞超微结构的改变;采用免疫组化法研究药物对信号转导和转录活化因子(signal transducer and activators of transcription,STAT)3及生存素(Survivin)基因表达的影响。结果鳖甲煎丸各组均可抑制肿瘤的生长,低、中、高3个组的抑瘤率分别为30.24%、36.72%和46.74%,并均可提高脾指数;环磷酰胺组抑瘤率为50.04%,脾指数增加不显著。光镜下观察发现,药物致使细胞排列稀疏,组织产生大量空泡。透射电镜观察可见,鳖甲煎丸组细胞发生凋亡,核固缩,线粒体结构变化。鳖甲煎丸各组STAT3、Survivin蛋白表达下调,与模型组相比差异显著。结论鳖甲煎丸体内具有抗肝癌移植瘤的作用,作用机制可能与其增强机体免疫功能,并诱导细胞凋亡有关,可能通过抑制STAT信号通路而发挥作用。Objective By detecting the morphology changes of H22 cells treated with Biejiajian Pill,to study its antitumor mechanism in vivo. Methods Murine hepatocarcinoma cells injected into ICR mice and then different doses of Biejiajian Pill（ 0. 411,1. 233,3. 699 g·kg^-1） were given. The dose of cyclophosphamide was 0. 03 g·kg^-1. Tumor growth inhibition rate and spleen index were measured. The changes of cell morphology were observed by lighting microscopy and transmission electron microscopy（ TEM）. The effects of STAT3 and Survivin gene expression were detected by immunohistochemical method. Results The tumor growth was inhibited by different doses of Biejiajian Pill and tumor growth inhibition rates were 30. 24%,36. 72% and 46. 74%.Moreover,spleen index increased. The growth rate of cyclophosphamide was 50. 04%,but spleen index didn＇t significantly increase. Observing by lighting microscopy,drugs caused cell lineage sparse and lots of vacuoles produced in tissue. Cell apoptosis,nucleus pycnosis,and mitochondrion structure disruption were observed by TEM. The STAT3 and Survivin protein of Biejiajian Pill groups down- regulated significantly,compared with model groups. Conclusion Biejiajian Pill has antitumor effect in vivo,which is probably related to enhance the body＇s immune function and induce cell apoptosis by inhibiting STAT signaling pathways.

关 键 词：鳖甲煎丸 H22肝癌 凋亡 STAT SURVIVIN 

分 类 号：R285.5[医药卫生—中药学]