炎症性肠病患者肠道菌群结构变化与炎性指标的关系研究  

Relationship between changes of intestinal microflora and inflammatory indexes in patients with inflammatory bowel diseases

作　　者：蔡尚党[1] 陈建设 娄宁[3] 彭冬先[4] 秦茂权[5] 蔡满地 

机构地区：[1]河南省中医院肛肠科,河南郑州450002 [2]中西医结合生殖中心,河南郑州450002 [3]中山大学附属肿瘤医院ICU,广东广州510060 [4]南方医科大学珠江医院妇产科,广东广州510280 [5]北京儿童医院血液科,北京100045 [6]河南大学医学院临床医学系,河南开封475004

出　　处：《中华医院感染学杂志》2016年第9期1942-1945,共4页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金青年基金资助项目(81001632)

摘　　要：目的研究炎症性肠病(IBD)患者肠道菌群结构变化与炎性指标的关系,为临床治疗提供病原学依据。方法选取2014年10月-2015年10月医院诊治的IBD活动期患者151例为研究对象,其中溃疡性肠炎(UC组)106例,克罗恩病(CD组)45例,根据患者的4项炎性指标检测结果继续划分亚组,将≤1项指标水平高于正常范围者作为炎性对照组,共58例,≥2项指标水平高于正常范围者作为高炎性指标组,共93例,另选同期医院体检的健康志愿者50名作为对照组,对所有受试人员的肠道菌群进行培养分析,研究炎症指标和菌群数目间的相关性;采用SPSS16.0软件进行统计分析。结果 UC组肠球菌属、酵母菌属、拟杆菌、双歧杆菌、消化球菌、乳杆菌属及小梭菌的数目多于对照组,真杆菌的数目少于对照组,差异有统计学意义(P<0.05);CD组肠球菌属、酵母菌属、拟杆菌、双歧杆菌、消化球菌及乳杆菌属的数目显著多于对照组,真杆菌及肠杆菌属的数目显著少于对照组,差异有统计学意义(P<0.05);UC组拟杆菌、双歧杆菌、消化球菌及乳杆菌属的培养阳性率显著高于对照组,差异有统计学意义(P<0.05);CD组酵母菌属、双歧杆菌、消化球菌及乳杆菌属的培养阳性率显著高于对照组,真杆菌的培养阳性率显著低于对照组,差异有统计学意义(P<0.05)。结论 IBD患者的肠道内肠球菌、真杆菌等菌群结构变化与之炎性指标之间存在一定程度的相关性,临床治疗时应予以重视。OBJECTIVE To study the relationship between the changes of intestinal microflora and the inflammatory indexes in patients with inflammatory bowel disease（IBD）so as to provide etiological basis for clinical treatment.METHODS A total of 151 patients with IBD in active stage who were treated in hospitals from Oct 2014 to Oct2015were recruited as the study objects,including 106 cases of ulcerative colitis（the UC group）and 45 cases of Crohn′s disease（the CD group）;the patients continued to be divided into subgroups according to the results of test of four inflammatory indexes,totally 58 patients with the level of no more than 1index higher than the normal range were assigned as the inflammatory control group,and 93 patients with the levels of no less than 2indexes higher than the normal range were set as the high inflammatory index group;meanwhile,50 healthy people who received the physical examination were chosen as the control group.The intestinal flora were cultured for all of the subjects,the correlation between the inflammatory indexes and the number of flora was observed,and the statistical analysis was performed with the use of SPSS16.0software.RESULTS The number of Enterococcus,Saccharomyces,Bacteriodes,Bifidobacterium,Peptococcus,Lactobacillus,or Clostridium was larger in the UC group than in the control group,while the number of eubacterium was less in the UC group than in the control group,and there was significant difference（P〈0.05）.The number of Enterococcus,Saccharomyces,Bacteriodes,Bifidobacterium,Peptococcus,or Lactobacillus was significantly larger in the CD group than in the control group,while the number of Eubacteriumor Enterobacter was significantly less in the CD group than in the control group（P〈0.05）.The positive rate of culture of Bacteriodes,Bifidobacterium,Peptococcus,or Lactobacillus was significantly higher in the UC group than in the control group（P〈0.05）.The positive rate of culture of Saccharomyces,Bifidobacterium,Peptococcus,or Lactobacillus was signific

关 键 词：炎症性肠病 肠道菌群 结构变化 炎性指标 相关性 

分 类 号：R378.2[医药卫生—病原生物学]