溶剂性质对两亲性聚肽静电纺丝的影响  

Effect of Solvent Properties on Electrospinning of Amphiphilic Polypeptides

作　　者：路亚亮 张帆[1] 陈涛[1] 胡莎妮 寇文娟[1] 罗雪莉[1] 王立权[1] 蔡春华[1] 

机构地区：[1]华东理工大学材料科学与工程学院上海市先进聚合物材料重点实验室超细材料制备与应用教育部重点实验室,上海200237

出　　处：《高分子学报》2016年第5期650-658,共9页Acta Polymerica Sinica

基　　金：中央高校基本科研业务费专项资金(项目号22A201514001);上海市大学生创新创业训练计划(项目号x14061)资助

摘　　要：通过对聚(γ-苄基L-谷氨酸酯)(PBLG)的亲水改性制备了两亲性聚(γ-苄基L-谷氨酸酯-co-羟乙谷酰胺)无规共聚肽(PBHG)用于静电纺丝制备超细纤维.通过傅里叶变换红外光谱、核磁共振氢谱表征了聚合物结构.通过测定溶液表面张力、黏度、电导率及扫描电镜观察纤维形貌考察了不同溶剂及PBHG浓度对纺丝溶液性质及电纺纤维的影响.通过水浸实验及MTT法评价了电纺纤维膜的亲水性及细胞相容性.研究发现在三氯甲烷(TCM)和四氢呋喃(THF)中PBHG采取α-螺旋构象,刚性分子链自取向排列,可获得直径为微米或亚微米的电纺纤维.以TCM为溶剂时,因溶液表面张力大、导电率低导致纤维品质较差,而以THF为溶剂可获得表面光洁、尺寸均匀的电纺纤维.当溶剂为三氟乙酸(TFA)时,PBHG采取无规线团构象,柔性分子链彼此缠结,同时溶液表面张力小、黏度低、电导率高,可获得纳米电纺纤维.但因TFA挥发性相对较差,易造成纤维粘连.将TFA与TCM复配后作为溶剂可改善纤维粘连问题.与PBLG电纺纤维相比,改性后的PBHG电纺纤维的亲水性得到了改善,可在水中保持纤维骨架而无需交联,并表现出良好的细胞相容性,能促进细胞在电纺纤维膜上的增殖.An amphiphilic random copolypeptide,poly（ γ-benzyl-L-glutamate-co-N-（ 2-hydroxyethyl）-Lglutamine]（ PBHG）,was modified from hydrophobic poly（ γ-benzyl-L-glutamate）（ PBLG） to improve hydrophilicity and applied to electrospin into ultrafine fibers. The molecular structure was confirmed by Fourier transform infrared spectroscopy（ FTIR） and proton nuclear magnetic resonance spectroscopy（1H-NMR）. The effects of solvent type and PBHG concentration on the properties of spinning solutions were investigated by measuring surface tension,viscosity and conductivity of the solutions. The morphologies of electrospun fibers were observed by scanning electron microscope. The hydrophilicity and cell proliferation ability of PBHG electrospun fibrous mats were also evaluated by water immersion and MTT assay,respectively. It was found that PBHG takes rigid α-helix conformation and tends to self-orientate in trichloromethane（ TCM） and tetrahydrofuran（ THF） from which micron or submicron fibers could be electrospun. The fibers electrospun from PBHG / TCM solutions have poor quality due to large surface tension,high viscosity and low conductivity of the solutions,whereas those obtained from PBHG / THF solutions have smooth surface and homogeneous diameter. In trifluoroacetic acid（ TFA）,PBHG molecules take random coil conformation and physically entangle with each other. The low surface tension,low viscosity and high conductivity of PBHG / TFA solutions are beneficial for electrispinning nano-fibers,but the low volatility of TFA leads to fiber adhesion. It can be alleviated by using TFA / TCM binary mixtures with suitable TFA content as solvent. PBHG electrospun fibers proved to be more hydrophilic than that of PBLG,and could maintain the skeleton in the water without further crosslinking. MTT results confirmed that PBHG fibers are non-cytotoxic and can promote the cell proliferation.

关 键 词：静电纺丝 聚(γ-苄基L-谷氨酸酯) 聚羟乙谷酰胺 溶液性质 构象 

分 类 号：TQ340.64[化学工程—化纤工业]