电针预处理对促排卵大鼠卵巢功能及VEGF的影响  

Effects of electroacupuncture pretreatment on ovarian function and expression of VEGF in rats with ovulation induction

作　　者：罗玺[1] 李茜[2] 程洁[2] 吕凯露[2] 华启新[2] 夏有兵[1,2] 

机构地区：[1]南京医科大学,江苏南京210029 [2]南京中医药大学,江苏南京210023

出　　处：《中国针灸》2016年第5期505-511,共7页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金面上项目:81473767;国家自然青年基金项目:81403481;江苏省高校面上课题:14KJB360003

摘　　要：目的:探讨电针预处理对促排卵大鼠卵巢功能的保护作用。方法:30只SD雌性大鼠按随机数字表编号,阴道涂片为动情期则为正常组,共10只,按照1∶1比例与雄性SD种鼠合笼。计算机产生随机序列号将剩余雌鼠分为促排模型组和电针预处理组,每组10只。促排模型组与电针预处理组采用孕马血清促性腺激素(pregnant mare serum gonadotropin,PMSG)和人绒毛膜促性腺激素(human chorionic gonadotropin,HCG)制造超数排卵模型,电针预处理组在造模与合笼前连续7d电针"关元""三阴交",每次15min,正常组与促排模型组不予其他干预。3组均于合笼后第3d晚23:00球后静脉取血,检测血浆雌二醇(E_2)、孕酮(P)的浓度。脱颈处死动物,留取卵巢组织一侧用HE染色法观察形态学改变,用免疫组织化学法检测各组卵巢组织中血管内皮生长因子(VEGF)及其受体VEGFR-2的表达;另一侧采用实时定量PCR技术测定各组卵巢组织中VEGF及VEGFR-2的基因表达水平。结果:电针预处理组大鼠卵巢发育卵泡的个数多于促排模型组和正常组(均P<0.05),单位面积内黄体面积占卵巢总面积的比值低于促排模型组(P<0.01)。电针预处理组大鼠血浆E2与P的比值趋于正常组(P<0.05),低于促排模型组(P<0.01);VEGF及VEGFR-2在卵巢黄体颗粒细胞及卵泡液中的蛋白表达均低于促排模型组(P<0.05);卵巢组织中VEGF、VEGFR-2的基因水平均低于促排模型组(P<0.05,P<0.01)。结论:电针预处理对促排卵后大鼠的卵巢功能具有一定的保护作用,其机制可能与VEGF及其相关受体参与调节有关。Objective To explore the protective effect of electroacupuncture（EA）pretreatment on ovarian function in rats with ovulation induction.Methods Thirty SD female rats were numbered according to random number table.According to vaginal smear method,rats of estrus were divided into a normal group（10rats）and cohabitated with male SD rats with the proportion of 1∶1.With computer-generated random number,the remaining rats were divided into a model group and an EA group,10 rats in each one.The model of superovulation was established with pregnant mare serum gonadotropin（PMSG）and human chorionic gonadotropin（HCG）in the model group and EA group.Before model establishment and cohabitation,rats in the EA group were treated with EA at＂Guanyuan（CV 4）＂and＂Sanyinjiao（SP 6）＂,once for 15 min,for consecutive 7 days.Rats in the normal group and model group received no further treatment.The third day 23：00 pm after cohabitation,blood samples in three groups were collected to test the level of estradiol（E_2）and progesterone（P）.After the rats were sacrificed,the HE staining method was applied to observe the morphological changes of ovarian tissue;the immunohistochemical method was applied to measure the expression of vascular endothelial growth factor（VEGF）and its receptor VEGFR-2;the real-time quantitative PCR technique was applied to measure the gene expression of VEGF and VEGFR-2.Results The number of ovarian follicle in the EA group was higher than that in the model group and normal group（all P〈0.05）;the ratio of corpus luteum size to ovarian size in the EA group was lower than that in the model group（P〈0.01）.The ratio of plasma estradiol to progesterone in the EA group tended to be normal group（P〈0.05）and lower than that in the model group（P〈0.01）.The protein expression of VEGF and VEGFR-2 in lutein granulosa cell and follicular fluid in the EA group was lower than that in the model group（P〈0.05）;gene level of VEGF and VEGFR-2 in ovarian tissue in the EA

关 键 词：电针 超数排卵 卵巢功能 血管内皮生长因子(VEGF) 

分 类 号：R246.3[医药卫生—针灸推拿学]