电针结合吗啡对骨癌痛大鼠痛觉敏化及脊髓星形胶质细胞的活化作用  

Effects of electroacupuncture and morphine on hyperalgesia and activation of spinal astrocyte in rats with bone cancer pain

作　　者：陆琪赟 陈颢[2] 崔光卫 王凡[1] 章婷婷[1] 蒯乐[1] 

机构地区：[1]上海中医药大学针灸推拿学院,上海201203 [2]复旦大学附属肿瘤医院中西医结合科,上海200032

出　　处：《上海中医药杂志》2016年第5期77-82,共6页Shanghai Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81202751);上海市卫计委加快中医药事业发展三年行动计划项目(ZY3-CCCX-3-3031);上海中医药大学预算内项目(2015YSN11)

摘　　要：目的观察电针结合吗啡对骨癌痛大鼠痛觉敏化及脊髓星形胶质细胞的活化作用。方法将60只雌性Wistar大鼠随机分为5组,每组12只。模型组、吗啡组、电针组和电针吗啡组大鼠右侧胫骨注入Walker 256大鼠乳腺癌细胞制备骨癌痛模型,假手术组大鼠注入Hanks液作为对照。造模后第15 d起,分别予以相应的电针和(或)吗啡干预,每天1次,连续6天。治疗前后,检测大鼠热刺激缩爪潜伏期(PWL),并采用Western blot法和免疫荧光组织化学法分别测定脊髓背角胶质原纤维酸性蛋白(GFAP)、白细胞介素1β(IL-1β)和神经生长因子(NGF)的表达情况。结果 1吗啡组、电针组和电针吗啡组大鼠的PWL均高于模型组(P<0.01),电针吗啡组PWL高于吗啡组与电针组(P<0.01)。2与模型组比较,电针组和电针吗啡组脊髓GFAP和NGF表达显著降低(P<0.01);吗啡组、电针组和电针吗啡组脊髓IL-1β表达显著降低(P<0.01)。结论电针结合吗啡可抑制骨癌痛大鼠痛觉敏化,其镇痛机制是能抑制脊髓背角星形胶质细胞的活化。Objective To observe the effects of electreacupuncture and morphine on hyperalgesia and the activation of spinal astrocyte in rats of bone cancer pain. Methods Sixty Wistar rats were randomly divided into sham operation group, model group, morphine group, electroacupunture group and eleetroaeupuncture with morphine group, 12 rats in each group. From the 15th day after establishing models （inoculating rat mammary gland carcinoma cell Walker 256 into marrow cavity of tibia） , the rats were treated accordingly by eleetroacupuncture and/or morphine once a day for 6 days. Paw withdrawal latency （PWL） was observed after treatments. Expressions of spinal GFAP, IL-113 and NGF were determined by Western blot method and immunofluorescent histochemical staining method. Results （~）PWL was longer in the three treatment groups than in the model group （ P 〈 O. O1 ）, with longer PWL in electroacupuncture and morphine group than in morphine group and electreacupunture group （ P 〈 O. O1 ）. @）Compared with the model group, expressions of spinal GFAP and NGF in electroaeupunture group and electreacupuncture with morphine group were reduced （ P 〈 0.01 ） ; expressions of spinal IL- 113 in the three treatment groups were all reduced （ P 〈 0.01 ）. Conclusion Eleetreacupuncture with morphine is effective to suppress hyperalgesia in rats of bone cancer pain by inhibiting the activation of spinal astrecyte.

关 键 词：电针 针药结合 癌症疼痛 针刺镇痛 星形胶质细胞 

分 类 号：R245[医药卫生—针灸推拿学]