纹党参多糖联合环磷酰胺对S_(180)荷瘤小鼠抗肿瘤增效机制的研究  

Study on Synergetic Mechanism of Polysaccharide Extracted from WenXian DangShen Combined with Cyclophosphamide in Fighting against S_(180) Tumor of the Mice

作　　者：吴红梅 苟于强 胡林海[3] 胡芳弟[4] 陈嘉屿[5] 杨春霞[4] 白瑞斌[4] 

机构地区：[1]甘肃省中医学校,甘肃兰州730050 [2]兰州军区疾病预防控制中心 [3]嘉峪关市第一人民医院 [4]兰州大学药学院 [5]兰州军区兰州总医院消化科

出　　处：《西部中医药》2016年第4期17-21,共5页Western Journal of Traditional Chinese Medicine

基　　金：甘肃省自然科学基金资助项目(编号1308RJZA101);甘肃省中医药科学技术研究资助项目(编号GZK-2011-73)

摘　　要：目的:研究纹党参多糖对环磷酰胺抗肿瘤作用的增效效应及作用机制。方法:选用健康成年昆明种小鼠135只,随机分为9组:正常对照组(ZD组),荷瘤对照组(HD组),环磷酰胺组(CP组),纹党参多糖高(WCPP-G组)、中(WCPP-Z组)、低(WCPP-D组)剂量治疗组及WCPP-G、WCPP-Z、WCPP-D联合CP组,每组15只。除ZD组小鼠外,将0.2 mL(1×10^(10)个/L)肿瘤细胞接种于各组小鼠腋窝皮下。ZD组和HD组给予0.5 m L生理盐水灌胃,6小时后给予等体积蒸馏水,1次/d;CP组0.5mL生理盐水灌胃,6小时后腹腔注射环磷酰胺20 mg/(kg·d);WCPP-G、WCPP-Z、WCP-D组分别给予高[400 mg/(kg·d)]、中[200 mg/(kg·d)]、低[100 mg/(kg·d)]浓度0.5 mL党参多糖水溶液灌胃,6小时后腹腔注射0.5 mL生理盐水;WCPP-G、WCPP-Z、WCP-D联合CP组分别给予高、中、低浓度0.5 m L党参多糖水溶液灌胃,6小时后腹腔注射环磷酰胺20 mg/(kg·d)。20天后计算抑瘤率,测定免疫相关指标及血清细胞因子IL-2、IL-6、IL-1β、IL-4、INF-γ、TNF-α的含量。结果:与ZD组比较,HD组IL-2、IL-6、TNF-α、INF-γ水平下降,其中TNF-α变化不明显,差异无统计学意义(P>0.05),其余各项变化明显,差异有统计学意义(P<0.05)。与HD组比较,CP组IL-2、IL-1β、IL-6、IL-4、TNF-α水平降低,其中INF-γ、IL-2水平变化不明显,差异无统计学意义(P>0.05),其余各项变化明显,差异有统计学意义(P<0.05);IL-2、IL-6、IL-1β、IL-4、TNF-α、INF-γ水平各剂量WCPP组及其联合CP组均显著增高,差异有统计学意义(P<0.05)。与HD组及CP组比较,WCPP-G、Z组及各剂量WCPP联合CP组IL-2、IL-6水平变化明显差异有统计学意义(P<0.05),各剂量WCPP组及其联合CP组IL-1β和TNF-α水平均有明显变化,差异有统计学意义(P<0.05)。与CP组比较,WCPP-D组IL-2水平变化明显,差异有统计学意义(P<0.05);WCPP-Z、WCPP-D组IL-4水平变化明显,差异有统计学意义(P<0.05)。与HD组比较,各剂量WCPP组及其联合CP组IL-4和INF-γ�hbstrt^g Objective： To study the synergy between the polysaccharide extracted from WenXian DangShen [Codonopsis pilosula. Var . modesta （Nannf） L. T. shen] and cyclophosphamide fighting against tumor and its mech- anism. Methods： All 135 mice were randomized into nine groups： normal control group （ZD group）, cancer-beating control group （HD group）, cyclophosphamide group（CP group）, the treatment groups ofhigh（WCPP-G group）, mod-erate（WCPP-Z group） and low（WCPP-D group） doses of the polysaccharide extracted from WenXian DangShen, and WCPP-G, WCPP-Z and WCPP-D combined with CP groups, 15 mice each group. Except the mice in ZD group, 0.2mL（lxl01~/L） tumor cells were transplanted under the skin of the armpit of the mice in different groups. ZD group and HD groups were given with intragastric administration of 0.5mL physiological saline, and the distilled water in the same amount in six hours, once per day; CP group accepted intragastric administration of 0.5mL physiological saline, and peritoneal injection of cyclophosphamide 20 mg/ （kg＇d） in six hours; WCPP-G, WCPP-Z and WWCP-D groups accepted intragastric administration ofpolysaccharide aqueous solution in high [400 mg/ （kg·d）], moderate [200 mg/（kg·d）] and low [100 mg/（kg·d）] concentrations, and peritoneal injection of 0.5mL physiological saline in six hours; WCPP-G, WCPP-Z and WWCP-D combined with CP groups received 0.5mL polysaccharide aqueous so- lution in high, moderate and low concentrations by lavage respectively, and peritoneal injection of cyclophos- phamide 20mg/ （kg·d） in six hours; tumor inhibition rate was calculated in 20 days, the contents of immune-related indexes, serum cytokines including IL-2, IL-6, IL- 1/3, IL-4, INF-T and TNF-a were detected. Results： Compared with ZD group, the levels of IL-2, IL-6, INF-T and TNF-a decreased, while the changes of TNF-a changed indistinctly in HD group （P〉0.05）, and others changed evidently （P〈0.05）. Compared with HD group, the levels

关 键 词：纹党参多糖 环磷酰胺 抗肿瘤 增效 

分 类 号：R285.5[医药卫生—中药学]