‘西伯利亚’百合单萜释放与Li-mTPS表达日变化动态分析（英文）  

Diurnal Changes of Monoterpenes Emission and Li-mTPS Expression in Lilium ‘siberia'

作　　者：李天娇[1] 胡增辉[1] 郑健[1] 冷平生[1] 杨凯[2] 

机构地区：[1]北京农学院园林学院/北京乡村景观规划设计工程技术研究中心/城乡生态环境北京实验室,北京102206 [2]北京农学院植物科学技术学院,北京102206

出　　处：《西北农业学报》2016年第5期763-769,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：The Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality(No.IDHT20150503);National Natural Science Foundation of China(No.31071817);Science and Technology Project of Beijing Municipal Education Commission(No.PM2014014207);Building Project of Beijing Laboratory of Urban and Rural Ecological Environment(No.PXM2015-014207-000014)~~

摘　　要：使用动态顶空法采集‘西伯利亚’百合不同时间点(7:30、9:30、11:30、13:30、15:30、17:30、19:30)释放的香气,采用自动热脱附-气相色谱/质谱联用(ATD-GC/MS)技术分析鉴定花香成分及释放量。结果表明:单萜释放量呈现先增后减的规律,11:30、13:30、15:30时的释放量较高。在所有的花香成分中,罗勒烯、芳樟醇和β-月桂烯的释放量最高,它们是‘西伯利亚’百合主要的单萜花香化合物。进一步研究发现,‘西伯利亚’百合花瓣中的Li-mTPS(一种百合单萜合成酶基因)的表达与单萜释放表现相似的规律。可见,Li-mTPS的表达在‘西伯利亚’百合主要的单萜释放中起着关键作用,而调控机制有待于进一步研究。Monoterpenes are an important category of volatile compounds in the floral scent. In this study, using dynamic headspace method, the floral scent emitted from Lilium ＇siberia＇ flowers was collected at different time points （7：30,9：30,11：30,13：30,15：30,17：30,19：30） during the daytime, then the components and release amounts were identified and analyzed by the automated thermal desorption-gas chromatography/mass spectrometry （ATD-GC/MS） technique. We found that release amounts of monoterpenes first increased and then decreased during the daytime, and a high level occurred at 11：30, 13：30, and 15：30. The high release amounts of ocimene, linalool, and β-myrcene indicated that they were the dominant monoterpenes in the floral scent of LiZium ＇siberia＇. In the following study, the daytime expression of Li-mTPS （a Lilium monoterpene synthase gene） in tepals was examined. Li-mTPS expression showed a similar pattern with monoterpene emission. The results showed that the Li-mTPS expression may play a key role in monoterpene emission from Lilium ＇siberia＇ flowers, but the mechanism needs to be investigated in the future study.

关 键 词：'西伯利亚’百合 花香 单萜 Li-mTPS表达 

分 类 号：S682.29[农业科学—观赏园艺]