HPLC法测定复方依沙吖啶软膏中乳酸依沙吖啶的含量  

Content Determination of Ethacridine Lactate in Compound Ethacridine Ointment by HPLC

作　　者：刘俊丽[1] 田佳懿[1] 段松冷[1] 曾蔚欣[1] 金锐[1] 孙路路[1] 

机构地区：[1]首都医科大学附属北京世纪坛医院药剂科,北京100038

出　　处：《中国药房》2016年第15期2109-2111,共3页China Pharmacy

基　　金：首都卫生发展科研专项项目(No.2011-2008-01)

摘　　要：目的：建立测定复方依沙吖啶软膏中乳酸依沙吖啶含量的方法。方法：采用高效液相色谱法。色谱柱为Agilent ZORBAX SB-C18,流动相为含0.1%辛烷磺酸钠溶液的磷酸盐缓冲液-乙腈（70∶30,V/V）,流速为1.0 ml/min,检测波长为270 nm,柱温为30℃,进样量为10μl。结果：乳酸依沙吖啶检测质量浓度线性范围为10.002-50.010μg/ml（r=0.999 9）;精密度、稳定性、重复性试验的RSD〈1%;回收率为98.96%-100.36%,RSD=0.49%（n=9）。结论：该方法简便、灵敏、重复性好,可用于复方依沙吖啶软膏中乳酸依沙吖啶的含量测定。OBJETCTIVE：To establish a method for the content determination of ethacridine lactate in Compound ethacridine ointment. METHODS：HPLC was performed on the column of Agilent ZORBAX SB-C18 with mobile phase of 0.1% Octanesulfonic acid sodium solution-acetonitrile（70 ∶ 30,V/V）at a flow rate of 1.0 ml/min,the detection wavelength was 270 nm,the column temperature was 30 ℃,and the injection volume was 10 μl. RESULTS：The linear range of ethacridine lactate was 10.002-50.010μ g/ml（r=0.999 9）;RSDs of precision,stability and reproducibility tests were less than 1%;recovery was 98.96%-100.36%（RSD=0.49%,n=9）. CONCLUSIONS：The method is simple,accurate and reproducible,and can be used for content determination of ethacridine lactate in Compound ethacridine ointment.

关 键 词：复方依沙吖啶软膏 乳酸依沙吖啶 含量测定 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]