HPLC法同时测定复方麝香草酚溶液中3种有效成分的含量  

Simultaneous Determination of 3 Effective Ingredients in Compound Thymol Solution by HPLC

作　　者：陈伟[1] 杨珺[1] 杨谨成[1] 周海燕[1] 王宇[1] 李国辉[1] 

机构地区：[1]中国医学科学院肿瘤医院药剂科,北京100021

出　　处：《中国药房》2016年第15期2119-2121,共3页China Pharmacy

摘　　要：目的：建立同时测定复方麝香草酚溶液中苯甲酸、水杨酸甲酯、麝香草酚含量的方法。方法：采用高效液相色谱法。色谱柱为Thermo Hypersil BDS C18,流动相为乙腈-水-冰醋酸（40∶60∶0.02,V/V/V）,流速为1 ml/min,检测波长为275 nm,柱温为30℃,进样量为10μl。结果：苯甲酸、水杨酸甲酯、麝香草酚检测质量浓度线性范围分别为0.045 2-0.903 0、0.049 2-0.984 4、0.046 4-0.927 0 mg/ml（r=0.999 9）;精密度、稳定性、重复性试验的RSD〈2%;加样回收率分别为98.40%-108.88%、98.21%-104.07%、96.83%-107.09%,RSD分别为3.26%、1.87%、3.33%（n=9）。结论：该方法简便、准确、特异性好,可用于同时准确测定复方麝香草酚溶液中苯甲酸、水杨酸甲酯、麝香草酚的含量。OBJECTIVE：To establish amethod for simultaneous determination ofbenzoic acid,methylis salicylas and thymol in Compound thymol solution.METHODS：HPLC was performed on the column of Thermo Hypersil BDS C18 with the mobile phase ofacetonitrile-water-acetic acid（40 ∶ 60 ∶ 0.02,V/V/V）at a flow rate of 1 ml/min,the detection wavelength was 275 nm,column temperature was 30 ℃,and injection volume was 10 μ l. RESULTS：The linear range was 0.045 2-0.9030 mg/ml for benzoic acid,0.0492-0.9844 mg/ml for methylis salicylas and 0.046 4-0.927 0 mg/ml for thymol;RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 98.40%-108.88%（RSD=3.26%,n=9）,98.21%-104.07%（RSD=1.87%,n=9）and96.83-107.09%（RSD=3.33%,n=9）,respectively. CONCLUSIONS：The method is simple,accurate and specific,and accurately determine the contents of benzoic acid,methylis salicylas and thymol in Compound thymol solution.

关 键 词：苯甲酸 水杨酸甲酯 麝香草酚 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]