不同浓度α-促黑素细胞激素对绵羊黑素细胞增殖和黑素生成的影响  

Effect of α-MSH on Proliferation and Melanogenesis in Sheep Melanocytes

作　　者：郝晓娟[1] 任玉红[1] 范瑞文[1] 张秋月[1] 曾庆宝[1] 武良琦 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801

出　　处：《中国生物化学与分子生物学报》2016年第5期578-586,共9页Chinese Journal of Biochemistry and Molecular Biology

基　　金：山西省科技攻关项目(No.20140311019-3);山西农业大学博士科研启动项目(No.XB2009022)资助~~

摘　　要：α-促黑素细胞激素(α-melanocyte-stimulating hormone,α-MSH)与黑皮质素1受体(melanocortin 1 receptor,MC1R)结合,通过c AMP信号通路影响黑色素的生成。然而,不同浓度α-MSH对绵羊黑色素生成的具体影响,还没有定论。本文旨在探讨不同浓度α-MSH对绵羊皮肤黑素细胞增殖和黑素合成的影响。实验组α-MSH(0、0.1、1、10、100、1 000 nmol/L)和空白对照组相比,荧光定量PCR和Western印迹检测出MC1R、小眼畸形相关转录因子(microphthalmia-associated transcription factor,MITF)和酪氨酸酶(tyrosinase,TYR),在添加10 nmol/Lα-MSH时,基因mRNA水平和蛋白水平的表达量最高,之后则降低;ELISA方法检测出c AMP含量总体增加,c GMP含量总体降低,添加10 nmol/Lα-MSH时,c AMP的产量增加最多,c GMP的产量降低最多;酶标法检测出10 nmol/Lα-MSH时,黑色素生成最多,之后则降低。上述结果证明,不同浓度α-MSH通过调控黑色素形成的c AMP路径,影响MC1R、MITF和TYR的基因和蛋白表达,从而影响黑色素的合成,以添加10 nmol/Lα-MSH时,对黑色素细胞表型和黑色素含量影响最为显著。α-Melanocyte-stimulating hormone （oL-MSH） combines with melanocortin 1 receptor （MC1R） to affect the production and distribution of melanin by cAMP pathway. However, the effects of different concentrations of α-MSH on the melanogenesis in sheep melanocytes have not been conclusive. Here we investigated the effects of α-MSH with different concentrations on the proliferation and melanin synthesis in sheep skin melanocytes. Real-time quantitative PCR and Western blot showed that gene mRNA and protein expression level of MC1R, microphthalmia-associated transcription factor （MITF） and myrosinase （TYR） was the highest in the melanocytes with 10 nmol/L supplementary, and then decreased, compared with that in the control. ELISA method detected that total yield of cAMP was increased, and the total yield of cGMP was decreased, and cAMP production increased and cGMP production declined with significant difference of 10 nmol/L. Enzyme labeled revealed that melanin synthesis was the highest in the melanocytes with 10 nmol/L supplementary, and then decreased. The results above suggested that α-MSH affected melanins production in melanocytes by regulating the cAMP pathway of melanogenesis with downstream of MC1R, MITF and TYR genes expression, and α-MSH of 10 nmol/L played the most important roles in the phenotype and melanogenesis of melanocytes.

关 键 词：α-促黑素细胞激素(α-MSH) 黑色素细胞 黑素皮质素1受体(MC1R) 小眼畸形相关转录因子(MITF) 酪氨酸酶(TYR) cAMP路径 

分 类 号：S852[农业科学—基础兽医学]