苹果组蛋白脱乙酰化酶基因HDA19的生物信息学分析与胁迫响应研究  

Bioinformation Analysis and Stress Response of a Histone Deacetylases Gene MdHDA19 in Apple

作　　者：张蕊芬[1] 刘会[1] 周李杰[1] 郭颖[1] 郝玉金[1] 由春香[1] 

机构地区：[1]山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,农业部黄淮地区园艺作物生物学与种质创制重点实验室,山东泰安271018

出　　处：《园艺学报》2016年第4期613-622,共10页Acta Horticulturae Sinica

基　　金：国家重点基础研究发展计划(‘973’计划)项目(2013CB945103);国家自然科学基金项目(31272142,31471854);教育部长江学者和创新团队发展计划项目(IRT15R42)

摘　　要：从‘嘎拉’苹果中克隆了MdHDA19(MDP0000132078)基因。它编码1个脱乙酰化酶,该基因ORF为1494bp,编码497个氨基酸,预测其蛋白质分子量为55.73kD,等电点为4.98。进化树分析表明,HDA19在不同物种间的氨基酸序列保守性较高,其中苹果MdHDA19与可可树TcHDA19亲缘关系最近(87.05%)。MdHDA19表达模式分析显示,其在苹果各个器官中均有表达,尤其在根和花中表达量较高。MdHDA19与苹果中MdSAT18互作,MdHDA19受到盐胁迫诱导,可能参与盐胁迫的调控。MdHDA19表达受低温(4℃)和高温(40℃)诱导,说明MdHDA19可能参与调控苹果对温度胁迫的响应。并用原核诱导的方法在体外表达了MdHDA19蛋白。Histone Deacetylases gene MdHDA 19 （MDPO000132078 ） was cloned from ＇ Gala＇ apple. Its ORF was 1 494 bp, which encoded 497 amino acids. The molecular mass of this protein was 55.73 kD, and pI was 4.98. The phylogenetic tree indicated that the HDA19 had sequence conservation among different species. MdHDA19 exhibited the highest sequence similarity with Theobroma cacao TcHDA19 （87.05%） . The MdHDA19 gene was expressed in all the tissues of apple, especially in root and flower. The MdHDA19 interacted with MdSAT18. The NaCI stress induces the expression level of MdHDA19. MdHDA19 might involve NaC1 stress response. The resulted showed that MdHDA19 gene was induced in low temperature （4 ℃ ） and high temperature （40℃ ） . In addition, the MdHDA19 recombinant protein was obtained. The results established the foundation for the further functions research of MdHDA19 protein.

关 键 词：苹果 脱乙酰化酶 MdHDA19 盐胁迫 温度 原核表达 

分 类 号：S661.1[农业科学—果树学]