藏族药甘青乌头脂溶性生物碱分析  

Fat-soluble Alkaloids from Aconitum tanguticum

作　　者：杨丽华[1,2] 李春[1] 林丽美[3] 王智民[1] 李钟[2] 

机构地区：[1]中国中医科学院中药研究所,中药质量控制技术国家工程实验室,北京100700 [2]广东药科大学,广州510006 [3]湖南中医药大学,长沙410208

出　　处：《中国实验方剂学杂志》2016年第10期32-36,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：北京市自然科学基金项目(7132152);中央级公益性科研院所基本科研业务费专项资金项目(ZZ070828)

摘　　要：目的：对藏族药甘青乌头全草的脂溶性生物碱进行研究。方法：采用多种现代色谱技术进行分离纯化,并通过理化性质、波谱分析（1D,2D-NMR和MS）,X射线单晶衍射等方法确定化合物结构。结果：从甘青乌头的脂溶性生物碱部位分离并鉴定了10个化合物,它们的结构分别为tanaconitine（1）,heteratisine（2）,11α,13α-dihydroxyhetisinone（3）,guan-fu base H（4）,β-谷甾醇（5）,苯甲酰胺（6）,邻苯二甲酸正丁基异丁基酯（7）,dihydroatisine（8）,hetisine（9）和对羟基苯乙醇（10）。结论：化合物1-4,8和9为二萜生物碱,化合物3,6-8为首次从甘青乌头中分离得到,本文首次对化合物3和4做了X射线单晶衍射数据分析。Objective： To study the chemical compositions of the fat-soluble alkaloids fraction of Aconitum tanguticum. Method： The fat-soluble alkaloids fraction was isolated and purified by using various modern chromatographic techniques,and the structures of the compounds were determined through physiochemical properties,spectral analysis（ 1D,2D-NMR and MS） and X-ray single crystal diffraction method. Result： Ten compounds were obtained from the fat-soluble alkaloids fraction of A. tanguticum, and their structures were identified as tanaconitine（ 1）,heteratisine（ 2）,11α,13α-dihydroxyhetisinone（ 3）, guan-fu base H（ 4）,β-sitosterol（ 5）,benzamide（ 6）,phthalic acid,butyl isobutyl ester（ 7）,dihydroatisine（ 8）,hetisine（ 9）and p-hydroxy phenylethanol（ 10）. Conclusion： Compounds 1-4, 8 and 9 were diterpenoid alkaloids;compounds 3,6-8 were isolated from the species for the first time. Moreover,the X-ray single crystal diffraction analysis of compounds 3 and 4 was done for the first time.

关 键 词：甘青乌头 化学成分 脂溶性生物碱 

分 类 号：R284.1[医药卫生—中药学]