高效液相色谱-三波长检测-梯度洗脱法同时测定橘红化痰丸中3种苷类成分  

Simultaneous Determination of Three Kinds of Glycosides Components in Juhong Huatan Wan by HPLC with Gradient Elution and Three Wavelength Detection

作　　者：梁咏倩 余积聪 宋粉云[1] 

机构地区：[1]广东药科大学药科学院,广州510006

出　　处：《中国实验方剂学杂志》2016年第10期80-83,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省自然科学基金项目(5002841)

摘　　要：目的:建立高效液相色谱-三波长检测-梯度洗脱法同时测定橘红化痰丸中3种苷类成分含量的方法。方法:采用Diamonsil-C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.1%磷酸水溶液进行梯度洗脱,流速1.0 m L·min^(-1),柱温30℃,进样量10μL,检测波长分别210 nm(苦杏仁苷),276 nm(甘草苷)和283 nm(柚皮苷)。结果:苦杏仁苷在25.0~175.0mg·L^(-1)具有良好的线性关系(r=0.999 1),苦杏仁苷平均回收率为101.5%,RSD 1.9%;甘草苷在2.0~50.0 mg·L^(-1)具有良好的线性关系(r=0.999 5),甘草苷平均回收率为100.8%,RSD 1.5%;柚皮苷在6.3~126.0 mg·L^(-1)具有良好的线性关系(r=0.999 2),柚皮苷平均回收率为101.7%,RSD 1.6%。结论:该法操作简单,重复性好,测定结果准确,可用于更好地控制该制剂的质量。Objective： A high performance liquid chromatographic method with gradient elution and three wavelength detection was developed for the simultaneous determination of amygdalin,liquiritin and naringin in Juhong Huatan Wan. Method： The separation was performed on a Diamonsil-C18 column with methanol and0. 1% phosphoric acid as the mobile phase of gradient elution at a flow rate of 1. 0 mL·min^-1. The detection wavelengths were set at 210,276,283 nm. Result： The linear range of amygdalin was 25. 0-175. 0 mg·L^-1（ r =0. 999 1）. The average recovery for amygdalin was 101. 5%,precision of the method was 1. 9%（ RSD）. The linear range of liquiritin was 2. 0-50. 0 mg·L^-1（ r = 0. 999 5）. The average recovery for liquiritin was 100. 8%,precision of the method was 1. 5%（ RSD）. The linear range of naringin was 6. 3-126. 0 mg·L^-1（ r = 0. 999 2）.The average recovery for naringin was 101. 7%,precision of the method was 1. 6%（ RSD）. Conclusion： The method is simple,accurate and reproducible. It is a good method for the quality control of the preparation.

关 键 词：橘红化痰丸 苦杏仁苷 甘草苷 柚皮苷 高效液相色谱法 三波长检测 梯度洗脱 

分 类 号：R284.1[医药卫生—中药学]