基于上转换发光纳米材料NaYF_4:Yb,Er的免疫层析试纸条测定血清中的降钙素原  

Determination of Procalcitonin in Serum with Immunochromatographic Strips Based on NaYF_4:Yb,Er Upconverting Nanophosphors

作　　者：朱瑾[1] 夏刚强[1] 罗芳[1] 雷丽江[1] 韩毓旺[1] 张红漫[1] 

机构地区：[1]南京工业大学化学与分子工程学院,南京211800

出　　处：《理化检验（化学分册）》2016年第5期501-505,共5页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金(21176124)

摘　　要：制备上转换发光纳米材料(UCP)——NaYF_4:Yb,Er,进行表面功能化修饰后将其作为荧光标记物,制备了快速测定血清中降钙素原(PCT)的免疫层析试纸条。通过优化得到免疫层析反应的最佳条件:抗体标记量为15mg·g^(-1),UCP免疫复合物的用量为40μg,检测线抗体划膜量为1.0μL·cm^(-1),孵育时间为5min。PCT的线性范围为0.05~50μg·L^(-1),检出限(3s/k)为0.020μg·L^(-1)。应用制得的免疫层析试纸条测定血清样品中的PCT,结果与罗氏电化学发光法的结果一致,测定值的相对标准偏差(n=3)小于23%。Upconverting nanophosphors（UCP）NaYF4：Yb,Er were synthesized and surface functional modified.The prepared UCP were taken as fluorescent marker in the immunochromatographic strips for rapid determination of procalcitonin（PCT）in serum.The optimized conditions for immunochromatographic reaction were as follow：15mg·g-1 of labeled antibody,40μg of immune complexe（UCP）,1.0μL·cm-1 of antibody zoned film on detection line and 5 min of incubation time.The linearity range of PCT was 0.05-50μg·L-1 with the detection limit（3s/k）of 0.020μg·L-1.The prepared immunochromatographic strips were used to determine PCT in serum,giving results in consistent with those obtained by Roche ECLIA and RSDs（n=3）less than 23%.

关 键 词：上转换发光纳米材料 免疫层析试纸条 降钙素原 血清 

分 类 号：O652.1[理学—分析化学]