银纳米粒子表面等离子共振吸收-分光光度法测定废水中的邻苯三酚  

Determination of Pyrogallol in Waste Water by Spectrophotometry Based on the Surface Plasmon Resonance Absorption of Silver Nanoparticles

作　　者：朱伟伟[1] 王齐[2] 黄昌才 张晖英[1] 

机构地区：[1]河池学院化学与生物工程学院,宜州546300 [2]昆明理工大学分析测试研究中心,昆明650000

出　　处：《理化检验（化学分册）》2016年第5期579-583,共5页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：2015广西高校科学研究项目(KY2015YB256);2014年云南省教育厅面上项目(KKJA201432042)

摘　　要：基于改良银镜反应,利用银纳米粒子的表面等离子共振吸收提出了测定废水中邻苯三酚的分光光度法。室温条件下,在氢氧化钠和氨水混合溶液的碱性环境中,以吐温-20作为分散剂,邻苯三酚作为还原剂,还原银氨溶液,反应生成银纳米粒子,溶液由无色变为亮黄色,并在410nm处产生银纳米粒子表面等离子共振吸收峰。溶液的吸光度与邻苯三酚的浓度在3.96×10-7~2.38×10^(-5) mol·L^(-1)范围内呈线性关系,检出限(3σ)为2.97×10-7 mol·L^(-1),摩尔吸光率为5.483×104L·mol^(-1)·cm^(-1)。该方法应用于工业废水中邻苯三酚的测定,测定值与普通分光光度法的测定结果一致,相对标准偏差(n=5)小于5.0%,加标回收率在95.4%~104%之间。A method for the determination of pyrogallol by spectophotometry was proposed based on the surface plasmon resonance absorption of the silver nanoparticles（AgNPs）that derived from modified silver mirror reaction.AgNPs were produced from the reduction of silver ammonia solution at room temperature in the media of sodium hydroxide and ammonia solution,with Tween-20 as dispersant and pyrogallol as reducing agent.The formation of AgNPs results in the colorless solution turned yellow with an absorption peak at 410 nm due to the surface plasmon resonance absorption of AgNPs.A linear relationship was found between the absorbance with the concentration of pyrogallol in the range of 3.96×10-7-2.38×10-5 mol·L-1,with detection limit（3σ）of 2.97×10-7 mol·L-1and the molar absorption coefficient of 5.483×104 L·mol-1·cm-1.The proposed method was applied to the determination of pyrogallol in industrial waste water,giving results in consistent with those obtained by normal spectrophotometry,with RSDs（n=5）less than 5.0% and recovery rates obtained by standard addition method in the range of 95.4%-104%.

关 键 词：分光光度法 邻苯三酚 银纳米粒子 工业废水 

分 类 号：O657.32[理学—分析化学]