共振散射光谱法测定水中痕量的过氧化氢  

Determination of Trace Hydrogen Peroxide in Water by Resonance Scattering Spectrometry

作　　者：唐宁莉[1] 单雅琦[1] 陈永宁[1] 蒙华毅[1] 

机构地区：[1]桂林理工大学化学与生物工程学院,桂林541004

出　　处：《理化检验（化学分册）》2016年第5期589-592,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金项目(21165008)

摘　　要：在pH4.3的柠檬酸-磷酸氢二钠缓冲溶液中,以牛血红蛋白为催化剂,加速过氧化氢与过量的KI反应生成I_3^-,I_3^-再与碱性染料乙基紫结合生成离子缔合物颗粒,大大增强了体系的共振散射强度,据此建立了一种测定水中痕量过氧化氢的共振散射光谱法。在659nm处,体系的共振散射强度增加值(ΔI)与过氧化氢的浓度在1.033×10^(-7)~2.272×10^(-6) mol·L^(-1)范围内呈线性关系,检出限(3s/k)为1.64×10-8 mol·L^(-1)。方法用于测定雨水中的过氧化氢,测定值的相对标准偏差(n=5)小于5.0%,加标回收率在104%~105%之间。In pH4.3 citric acid-disodium hydrogen phosphate buffer solution,bovine hemoglobin as a catalyst could accelerate the reaction rate of hydrogen peroxide oxidizing the excess KI to I_3-,and I_3- combined with basic dye ethyl violet to form ion association particles,which enhanced the resonance light scattering intensity greatly.Base on this,a new resonance scattering spectrometry was proposed for the determination of trace hydrogen peroxide in water samples.The increased resonance light scattering intensity（ΔI）at 659 nm was linear to the concentration of hydrogen peroxide in the range of 1.033×10-7-2.272×10-6 mol·L-1,with the detection limit（3s/k）of 1.64×10-8 mol·L-1.The method was applied to the determination of hydrogen peroxide in rain water samples,giving RSDs（n=5）less than 5.0% and recovery rates obtained by standard addition method in the range of 104%-105%.

关 键 词：共振散射光谱法 过氧化氢 水 牛血红蛋白 乙基紫 

分 类 号：O657.3[理学—分析化学]