丙烯酰胺对大鼠谷氨酸和γ-氨基丁酸神经递质含量的影响  

Study on changes of glutamate and γ-aminobutyric acid neurotransmitters induced by acrylamide in rats

作　　者：张斌[1] 肖经纬[1] 陈宵[1] 李忠生[1] 曹鹏[1] 朱丹[1] 李斌[1] 

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所毒理室/中国疾病预防控制中心化学污染物与健康重点实验室,北京100050

出　　处：《毒理学杂志》2016年第2期107-112,共6页Journal of Toxicology

基　　金：国家自然科学基金(81273110);国家科技支撑计划(2014BAI12B00);卫生行业专项(201402021)

摘　　要：目的探讨丙烯酰胺(ACR)染毒致大鼠大脑皮层和小脑谷氨酸(Glutamate,Glu)和γ-氨基丁酸(γ-aminobutyric acid,GABA)的含量变化。方法 60只雄性SD大鼠分为6组,即11 d试验组(生理盐水对照组、30 mg/kg ACR染毒组和50 mg/kg ACR染毒组)和21 d试验组(生理盐水对照组、15 mg/kg ACR染毒组和30 mg/kg ACR染毒组);步态评分评价神经行为改变;LC-MS/MS法测定染毒终点大脑皮层和小脑Glu和GABA含量变化。结果 11 d试验组在染毒终点时,50 mg/kg ACR染毒组与生理盐水对照组、30 mg/kg ACR染毒组相比,体重显著降低(P<0.05),步态评分显著增高(P<0.05);21d试验组在染毒终点时,30 mg/kg ACR染毒组与生理盐水对照组、15 mg/kg ACR染毒组相比,体重显著降低(P<0.05),步态评分显著增高(P<0.05);30 mg/kg 21 d和50 mg/kg 11 d染毒组与对照组相比,大脑皮层和小脑内兴奋性神经递质Glu含量显著降低(P<0.05),且降低幅度一致。结论 ACR引起大鼠大脑皮层和小脑内兴奋性神经递质Glu含量的降低,可能是导致其神经毒性的致病机制之一。Objective To explore the changes of neurotransmitter induced by acrylamide in rats. Methods Sixty SD male rats were randomly divided into 11 days test group （control group, 30 mg/kg ACR group, 50 mg/kg ACR group） and 21 days test group （control group, 15 mg/kg ACR group, 30 mg/kg ACR group）. Changes of weight and behavior were observed. Gait scores were noted. The levels of Glutamate and GABA were measured using LC-MS/MS. Results Weight of rats treated with 50 mg/kg ACR was decreased and neurobehavioral changes were increased significantly compared with the control group and 30 mg/kg ACR treatment group at the end of the test （P 〈 0. 05）. Weight of rats treated with 30 mg/kg ACR was decreased and neurobehavioral changes were increased significantly compared with the control group and 15 mg/kg ACR treatment group at the end of test （ P 〈 0.05 ）. The level of Glutamate in cerebral cortex and cerebellum was decreased significantly at 30 mg/kg group at the end of 21 d exposure period and 50 mg/kg group at the end of 11 d exposure period compared with the control group （ P 〈 0.05 ）. Conclusion The level reduction of Glutamate in cerebral cortex and cerebellum may be one of the mechanisms of ACR-induced neurotoxicity.

关 键 词：丙烯酰胺 神经递质 谷氨酸 Γ-氨基丁酸 

分 类 号：R114[医药卫生—卫生毒理学]