Vitrolife与Cook培养基对胚胎生长与妊娠结局的影响  

Influence of Vitrolife and Cook culture medium on embryo growth and pregnancy outcome

作　　者：宋冰冰[1] 谢娟珂[1] 韦多[1] 宋小兵[1] 张亚楠[1] 张翠莲[1] 

机构地区：[1]郑州大学人民医院河南省人民医院生殖医学中心,郑州450003

出　　处：《中华实用诊断与治疗杂志》2016年第5期443-445,共3页Journal of Chinese Practical Diagnosis and Therapy

基　　金：河南省科技厅重点科技攻关项目(122102310536)

摘　　要：目的比较Vitrolife培养基与Cook培养基对胚胎生长与妊娠结局的影响。方法行体外授精-胚胎移植技术治疗不孕患者1 441例,根据使用的培养基分为Vitrolife组914例和Cook组527例,比较2组移植日子宫内膜厚度、获卵数、卵子成熟度、两原核(two pronucleus,2PN)受精率、2PN卵裂率、取卵后第3天(D3)可利用胚胎率、移植胚胎数、种植率、临床妊娠率、早期流产率和异位妊娠率。结果Vitrolife组移植日子宫内膜厚度[(11.08±2.56)mm]、获卵数[(9.23±4.00)枚]、卵子成熟度(85.56%)、2PN受精率(60.08%)、2PN卵裂率(98.73%)、D3可利用胚胎率(80.31%)与Cook组[(10.92±2.54)mm、(9.08±3.81)枚、85.68%、58.48%、98.46%、80.55%]比较差异均无统计学意义(P>0.05);对移植D3卵裂期胚胎,Vitrolife组移植胚胎数[(1.88±0.33)枚]、种植率(44.01%)、临床妊娠率(59.68%)、早期流产率(8.11%)、异位妊娠率(2.12%)与Cook组[(1.86±0.34)枚、45.03%、61.55%、8.74%、1.94%]比较差异均无统计学意义(P>0.05);对移植取卵后第5天(D5)、取卵后第6天(D6)囊胚,Vitrolife组平均移植胚胎数[(1.74±0.44)]枚、种植率(58.75%)、临床妊娠率(65.22%)、早期流产率(6.67%)、异位妊娠率(0)与Cook组[(1.76±0.44)枚、61.36%、68.00%、5.88%、5.88%]比较差异均无统计学意义(P>0.05)。结论 Vitrolife培养基与Cook培养基对胚胎生长与妊娠结局的影响无明显差异。Objective To compare the influence of Vitrolife culture medium and Cook culture medium on embryo growth and pregnancy outcome. Methods A total of 1 441 patients receiving in vitro fertilization and embryo transfer （IVF-ET） were divided into Vitrolife group （n= 914） and Cook group （n= 527） according to the medium used in IVF procedure. The endometrial thickness, average number of oocytes, oocyte maturation rate, two pronucleus （2PN） fertilization rate, 2PN cleavage rate, available embryo rate by day 3 after retrieving oocytes, number of transferred embryos, implantation rate, clinical pregnancy rate, early abortion rate and ectopic pregnancy rate were compared between two groups. Results There were no significant differences in the endometrial thickness （（11.08±2.56）, （10.92±2.54） mm）, the number of oocytes （9.23 ± 4. 00, 9.08 ± 3. 81）, the oocyte maturation rate （85. 56%, 85. 68%）, the 2PN fertilization rate （60.08%, 58.48%）, the 2PN cleavage rate （98.73%, 98.46%） and the available embryo rate by day 3 after retrieving oocytes （80.31%, 80.55%） between Vitrolife group and Cook group （P〉0.05）. There were no significant differences in the average number of transferred embryos （1.88±0.33, 1.86±0.34）, the implantation rate （44.01%, 45.93%）, the clinical pregnancy rate （59.68%, 61. 55%）, the early abortion rate （8.11%, 8.74%） and the ectopic pregnancy rate （2.12%, 1.94%） of the transferred cleavage stage embryo between Vitrolife group and Cook group （P〉0.05）. There were no significant differences in the average number of transferred embryos by day 5 and 6 after retrieving oocytes （1.74%. 44, 1. 76±0. 44）, the implantation rate （58. 75%, 61. 36%）, the clinical pregnancy rate （65. 22%,68.00%）, the early abortion rate （6. 67%, 5. 88%）, the ectopic pregnancy rate （0, 5. 88%） of the transferred blastosphere between Vitrolife group and Cook group （P〉0.05）. Conclusion There is no obvious difference between Vitrolif

关 键 词：Vitrolife培养基 Cook培养基 胚胎生长 妊娠 

分 类 号：R714.8[医药卫生—妇产科学]