低病毒载量时丙型肝炎病毒基因分型研究  

Hepatitis C virus genotyping in patients with lower hepatitis C virus RNA load

作　　者：奚琳琳[1] 靳秀[2] 蔡庆春[1] 丁瑞敏[1] 李威[2] 尚佳[2] 

机构地区：[1]河南中医药大学第三附属医院检验科,郑州450008 [2]郑州大学人民医院河南省人民医院感染科,郑州450003

出　　处：《中华实用诊断与治疗杂志》2016年第5期476-477,共2页Journal of Chinese Practical Diagnosis and Therapy

基　　金：河南省科技攻关项目(162102310030);河南省科技攻关项目(152102310135);河南省医学科技攻关普通项目(201303164)

摘　　要：目的探讨丙型肝炎病毒（hepatitis C virus，HCV）低病毒载量时，HCV核心蛋白（Core）和Core／包膜蛋白1（envelope protein 1，E1）区直接测序法对HCV基因分型的影响。方法HCVRNA 10^3-〈10^4拷贝／mL患者30例，采集其血清标本，采用巢式PCR对Core和Core／E1区分别扩增，产物直接测序，构建进化树进行基因分型。结果Core区扩增阳性25例（83．3％），其中1b型15例，2a型5例，3a型2例和6a型3例；tore／E1区扩增阳性19例（63．3％），其中1b型11例，2a型4例，3a型2例和6a型2例。结论当HCV—RNA载量较低时，Core区较Core/E1区有较高扩增效率和准确性，是临床或科研中应用直接测序法对HCV基因分型较理想的基因区。Objective To explore the influence of direct sequencing of Core and Core/envelope protein （El） regions on hepatitis C virus （HCV） genotyping in lower HCV-RNA load. Methods The serum samples from 30 patients with lower HCV-RNA load （HCV RNA 10^3-〈10^4 copies/mL） were collected to amplify Core and Core/E1 regions by nested PCR, and the subtypes were directly sequenced for genotyping. Results Among the 30 samples, 25 samples （83. 3%） were successfully typed by phylogenetic analysis of directly sequenced Core region, including 15 samples of type 1b, 5 samples of type 2a, 2 samples of type 3a and 3 samples of type 6a. And another 19 samples （63.3%） were positive in Cote/E1 region including 11 samples of type lb, 4 samples of 2a, 2 samples of 3a and 2 samples of 6a respectively. Conclusion Core has higher amplification efficiency and accuracy than Cote/El in patients with lower HCV RNA load, and is a favorable region for HCV genotyping in direct sequencing in clinical and scientific research.

关 键 词：丙型肝炎病毒 基因型 核心蛋白 包膜蛋白1 低病毒载量 

分 类 号：R440[医药卫生—诊断学] R512.63[医药卫生—临床医学]