TXNRD1基因多态性与乌珠穆沁绵羊生长性状的关联分析  

TXNRD1 Gene Polymorphisms and Its Association with Growth Traits in Ujumqin Sheep

作　　者：马晓萌[1] 轩俊丽[1] 王慧华[1] 袁泽湖[1] 吴明明[1] 朱才业[1] 刘瑞凿 魏彩虹[1] 赵福平[1] 张莉[1] 杜立新[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所

出　　处：《畜牧兽医学报》2016年第5期909-921,共13页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：中国农业科学院北京畜牧兽医研究所中央级公益性科研院所基本科研业务费专项资金项目(2014ywf-yb-7);国家"十二五"863项目"绵羊肉用性状全基因组关联分析研究"(2011AA100307-03)

摘　　要：本研究旨在探讨TXNRD1多态性与乌珠穆沁绵羊生长性状之间的关联性,以期找到与乌珠穆沁绵羊生长有关的分子标记。采用DNA混池测序技术筛选TXNRD1基因外显子突变位点,并采用质谱分型技术检测343头乌珠穆沁绵羊群体突变位点的基因型,并对其单个SNP及多个SNPs位点之间的组合基因型与生长性状之间进行关联分析。结果发现：在该基因第1外显子处发现1个C→T的错义突变（RS10）;在第10外显子发现1个A→C同义突变（RS17）;在第12外显子处发现1个T→C同义突变（RS18）。χ-2适合性检验表明,3个位点均处于HardyWeinberg平衡状态（P〉0.05）。连锁不平衡和单倍型分析表明：RS10-RS17,RS10-RS18两个位点之间可能存在强连锁,RS17-RS18之间弱连锁。CAT是优势单倍型,其频率为0.356。关联分析发现,每个位点的3种基因型的个体在4月龄体重和胸围上均有显著差异（P〈0.05）。RS10位点3种基因型的个体在6月龄体重、体高、胸围及胸宽上差异显著（P〈0.05）,RS17位点3种基因型的个体在6月龄体高上差异显著（P〈0.05）,RS18位点上3种基因型的个体在6月龄体重、体高、体斜长和胸围上有显著性差异（P〈0.05）。组合基因型关联分析发现,不同多态位点之间的组合基因型在乌珠穆沁绵羊不同生长性状之间差异显著（P〈0.05）。综上所述,可以将TXNRD1基因的多态性位点作为分子标记,在乌珠穆沁绵羊的选育中进行探索和尝试。The aim of this study was to investigate the association between the TXNRD1 gene polymorphisms and the sheep growth traits, and to find the molecular markers related to the growth traits of Ujumqin sheep. The variations in the exons of TXNRD1 gene were detected using DNA pool technology. Then Sequenom Mass-array technology was used to detect the geno- types at the mutation sites of the TXNRD1 gene in 343 Ujumqin sheep. The polymorphisms of TXNRD1 gene and its association with growth traits of Ujumqin sheep were analyzed by SPSS 22.0 software. The results showed that the C→T missense mutation （RS10） in exon 1 ,the A→C synonymous mutation（RS17） in exon 10 and the T→C synonymous mutation（RS18） in exon 12 were found,respectively. The x2 test showed that all the 3 sites were in Hardy-Weinberg equilib- rium（P〈0.05）. Then linkage disequilibrium and haplotypes were analyzed, the results showedthat RS10-RS17, RS10-RS18 were strongly linked, while RS17-RS18 were weakly linked. The dominant haplotype was CAT with a frequency of 0. 356. Assocition analysis suggested that 3 gen- otypes at each mutation site had significant difference in 4-month-age weight and chest girth （P（0.05）. The 3 genotypes of RS10 showed significant difference in 6-month-age weight, body height, chest girth and chest width（P〈0.05）. At kS17 site, the 3 genotypes also had significant difference in 6-month-age body height （P〈0. 05）. At RS18 site, there were significant difference among the 3 genotypes in 6- month-age weight, body height, body length and chest girth （P 〈0.05）. Meanwhile, the different com- bined genotypes showed significant difference in various Uj umqin growth traits （P〈0.05）. Therefore, the results suggest that TXNRD1 mutation sites maybe markers related to the Ujumqin sheep growth traits and can be used as candidate markers in Ujumqin sheep molecular breeding.

关 键 词：乌珠穆沁绵羊 TXNRD1基因 多态性 生长性状 关联分析 

分 类 号：S826[农业科学—畜牧学]