蛋白激酶CK2抑制剂对鸡朊蛋白高表达和抑制表达DF-1细胞生物学行为的影响  

Effects of CK2 Inhibitor on Biological Behaviour of DF-1 Cells with High Expression and Suppression Expression of Chicken PrP^C

作　　者：万学瑞[1] 杨润霞[1] 王川[1] 刘桂林[1] 吴润[1] 

机构地区：[1]甘肃农业大学动物医学院,兰州730070

出　　处：《畜牧兽医学报》2016年第5期985-992,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31160510);甘肃农业大学动物医学院教研产学支持计划(JYCX-KX009)

摘　　要：为探讨CK2在鸡细胞型朊蛋白(ChPrP^C)高表达和抑制表达的DF-1细胞增殖、黏附、侵袭和凋亡过程中的作用及其与ChPrP^C表达量的关系,以DF-1-PrP和DF-1-SiRNA-3细胞为模型,DF-1细胞为对照,分别用0、25、50、100nmol·L^(-1)米托蒽醌处理以抑制CK2,检测细胞对鼠尾胶原的黏附能力,Transwell小室法检测细胞侵袭力,MTT法检测细胞增殖,流式细胞仪检测细胞凋亡,RT-PCR法检测PRNP基因mRNA转录。结果显示,DF-1-PrP、DF-1-SiRNA-3和DF-1细胞的PRNP基因mRNA转录量随着米托蒽醌浓度的增加均减少,其增殖、黏附、侵袭能力相应下降,而总凋亡率均升高;但在同一米托蒽醌浓度下,DF-1-PrP细胞增殖、黏附、侵袭能力始终高于DF-1细胞,总凋亡率均低于DF-1细胞;DF-1-SiRNA-3细胞则相反。表明ChPrP^C的高表达可促进DF-1细胞增殖、黏附和侵袭,抑制其凋亡,而ChPrP^C的低表达则相反;CK2在ChPrP^C介导DF-1细胞增殖、黏附、侵袭和凋亡过程中具有重要的作用。本研究结果为进一步阐明ChPrP^C生理功能的分子机制奠定基础。In order to detect the effect of CK2 on DF-1 ceils proliferation,adhesion,invasion and apoptosis based on the higher and lower ChPrPc expression and its relationship, DF-1 cells with higher expression of chicken PrPc （DF-1-PrP）,DF-1 cells with suppression expression of chicken prpC（DF-1-SiRNA-3） and DF-1 cells were used as cell models,after they were treated with 0,25, 50,100 nmol · L-1 mitoxantrone, adhesion assay, transwell assay, MTT assay, flow cytometric as- say and RT-PCR analyses were used to detect cell adhesion,invasion, proliferation, apoptosis and transcription of PRNP mRNA,respectively. The data showed that the expression of PRNP mR- NA of DF-1-PrP,DF-1-SiRNA-3 and DF-1 cells were all decreased with the increasing of mitox- antrone concentration, and adhesion,invasion and proliferation ability were reduced, but apoptosis rate were increased. Furthermore, under the same mitoxantrone concentration, adhesion, invasion and proliferation ability of DF-1-PrP cells was higher than DF-1 cells,apoptosis rate was lower, but which of DF-1-SiRNA-3 cells was opposite. Results indicated that higher expression of ChPrPc promoted DF-1 cells adhesion,invasion and proliferation and inhibited apoptosis,but sup- pression expression of ChPrPc was opposite,CK2 plays an important role in those processes. Dataof this study lay the foundation for further clarify the molecular mechanism of ChPrPc physiologi- cal function.

关 键 词：蛋白激酶2 鸡朊蛋白 DF-1细胞系 米托蒽醌 凋亡 

分 类 号：S831.1[农业科学—畜牧学] S852.2[农业科学—畜牧兽医]